PerR functions as a redox-sensing transcription factor regulating metal homeostasis in the thermoacidophilic archaeon Saccharolobus islandicus REY15A.

Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE

Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.

Transcription Factors*/metabolism ; Transcription Factors*/genetics ; Transcription Factors*/chemistry ; Archaeal Proteins*/genetics ; Archaeal Proteins*/metabolism ; Archaeal Proteins*/chemistry ; Metals*/metabolism ; Sulfolobaceae*/genetics ; Sulfolobaceae*/metabolism; Homeostasis ; Oxidation-Reduction ; Oxidative Stress/genetics ; Gene Expression Regulation, Archaeal ; Reactive Oxygen Species/metabolism ; Hydrogen Peroxide/pharmacology ; Zinc/metabolism

Thermoacidophilic archaea thrive in environments with high temperatures and low pH where cells are prone to severe oxidative stress due to elevated levels of reactive oxygen species (ROS). While the oxidative stress responses have been extensively studied in bacteria and eukaryotes, the mechanisms in archaea remain largely unexplored. Here, using a multidisciplinary approach, we reveal that SisPerR, the homolog of bacterial PerR in Saccharolobus islandicus REY15A, is responsible for ROS response of transcriptional regulation. We show that with H2O2 treatment and sisperR deletion, expression of genes encoding proteins predicted to be involved in cellular metal ion homeostasis regulation, Dps, NirD, VIT1/CCC1 and MntH, is significantly upregulated, while expression of ROS-scavenging enzymes remains unaffected. Conversely, the expression of these genes is repressed when SisPerR is overexpressed. Notably, the genes coding for Dps, NirD and MntH are direct targets of SisPerR. Moreover, we identified three novel residues critical for ferrous ion binding and one novel residue for zinc ion binding. In summary, this study has established that SisPerR is a repressive redox-sensing transcription factor regulating intracellular metal ion homeostasis in Sa. islandicus for oxidative stress defense. These findings have shed new light on our understanding of microbial adaptation to extreme environmental conditions.
(© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.)

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32 393 973 National Natural Science Foundation of China; 2020YFA0906800 National Key Research and Development Program of China

0 (Transcription Factors)
0 (Reactive Oxygen Species)
0 (Archaeal Proteins)
0 (Metals)
BBX060AN9V (Hydrogen Peroxide)
J41CSQ7QDS (Zinc)

Date Created: 20241227 Date Completed: 20250428 Latest Revision: 20250428

20250429

PMC11724291

10.1093/nar/gkae1263

39727184