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STRUCTURING A SET OF OBJECTS SUCH AS CELLS AND MICRON-SIZED PARTICLES USING ACOUSTIC FORCE

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  • Publication Date:
    April 10, 2025
  • Additional Information
    • Document Number:
      20250115862
    • Appl. No:
      18/694769
    • Application Filed:
      September 27, 2022
    • Abstract:
      A technique for moving various objects such as cells and particles of hydrogel or a compressible material, suspended in a fluid, so as to form a layered structure akin to human organ tissue. A standing sound wave is propagated through the fluid so as to position the cells on a pressure node and the particles on a pressure antinode. As such, the cells have a positive acoustic contrast relative to the fluid, while the particles have a negative acoustic contrast relative to the fluid.
    • Assignees:
      CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (PARIS, FR), ECOLE SUPERIEURE DE PHYSIQUE ET DE CHIMIE INDUSTRIELLES DE LA VILLE DE PARIS (PARIS 05, FR), INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (PARIS, FR), SORBONNE UNIVERSITE (PARIS, FR), UNIVERSITE PARIS CITE (PARIS, FR)
    • Claim:
      1-9. (canceled)
    • Claim:
      10. A method for structuring a set of objects, comprising: arranging a fluid and said objects suspended in the fluid in a cavity, and generating a stationary acoustic wave in the cavity so as to produce an acoustic radiation force causing the objects to move in the cavity, wherein said objects comprise: first objects which have a positive acoustic contrast with respect to the fluid, and second objects which have a negative acoustic contrast with respect to the fluid, and the stationary acoustic wave is held so as to keep the objects thus moved in acoustic levitation.
    • Claim:
      11. The method according to claim 10, wherein the stationary acoustic wave generated in the cavity has a wavelength less than twice a dimension of the cavity along a direction of propagation of the stationary acoustic wave, preferably less than or equal to this dimension.
    • Claim:
      12. The method according to claim 10, comprising, after positioning the objects under the action of the acoustic radiation force, a step of introducing a substance into the cavity so as to form a matrix capable of supporting the first objects.
    • Claim:
      13. The method according to claim 12, wherein said substance comprises a hydrogel prepolymer.
    • Claim:
      14. The method according to claim 12, wherein the matrix is porous.
    • Claim:
      15. The method according to claim 12, wherein said substance comprises a photopolymerisable material, the method comprising, after introducing the substance into the cavity, a step of light stimulating the substance so as to polymerise it.
    • Claim:
      16. The method according to claim 10, comprising, after positioning the objects under the action of the acoustic radiation force, a step of incubating the objects.
    • Claim:
      17. The method according to claim 10, comprising, after positioning the objects under the action of the acoustic radiation force, a step of heating the second objects so as to melt them.
    • Claim:
      18. The method according to claim 10, comprising, after positioning the objects under the action of the acoustic radiation force, a step of encapsulating the first objects, this step comprising introducing third objects having a positive acoustic contrast with respect to the fluid into the cavity.
    • Current International Class:
      12; 12
    • Accession Number:
      edspap.20250115862