Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

The type I-E CRISPR-Cas system influences the acquisition of bla KPC -IncF plasmid in Klebsiella pneumonia .

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101594885 Publication Model: Print Cited Medium: Internet ISSN: 2222-1751 (Electronic) Linking ISSN: 22221751 NLM ISO Abbreviation: Emerg Microbes Infect Subsets: MEDLINE
    • Publication Information:
      Publication: 2019- : [Philadelphia, PA] : Taylor & Francis
      Original Publication: New York : NPG, 2012-2018.
    • Subject Terms:
      CRISPR-Cas Systems* ; Carbapenem-Resistant Enterobacteriaceae*/genetics ; Carbapenem-Resistant Enterobacteriaceae*/isolation & purification ; Gene Transfer, Horizontal*; Klebsiella pneumoniae/*genetics; Bacterial Proteins/genetics ; Bacterial Proteins/isolation & purification ; China/epidemiology ; Humans ; Klebsiella Infections/epidemiology ; Klebsiella pneumoniae/isolation & purification ; Molecular Epidemiology ; Plasmids/isolation & purification ; Pneumonia/epidemiology ; Pneumonia/microbiology ; beta-Lactamases/genetics ; beta-Lactamases/isolation & purification
    • Abstract:
      Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and bla KPC -IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of bla KPC -IncF plasmids are favored by CG258 K. pneumoniae, it was of interest to examine the co-distribution of CRISPR and bla KPC -IncF plasmids in such isolates. We collected 459 clinical K. pneumoniae isolates in China and collected 203 global whole-genome sequences in GenBank to determine the prevalence of CRISPR-Cas systems. We observed that CRISPR-Cas system was significantly scarce in the CG258 lineage and bla KPC -positive isolates. Furthermore, the results of conjugation and plasmid stability assay fully demonstrated the CRIPSR-Cas system in K. pneumoniae could effectively hindered bla KPC -IncF plasmids invasion and existence. Notably, most bla KPC -IncF plasmids were also proved to be good targets of CRISPR owing to carry matched and functional protospacers and PAMs. Overall, our work suggests that type I-E CRISPR-Cas systems could impact the spread of bla KPC in K. pneumoniae populations, and the scarcity of CRISPR-Cas system was one of potential factors leading to the propagation of bla KPC -IncF plasmids in CG258 K. pneumoniae .
    • References:
      Gene. 2002 May 15;290(1-2):153-61. (PMID: 12062810)
      Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10098-103. (PMID: 21646539)
      Mol Microbiol. 2010 Sep;77(6):1367-79. (PMID: 20624226)
      Trends Microbiol. 2014 Dec;22(12):686-96. (PMID: 25304194)
      Antimicrob Agents Chemother. 2017 Jun 27;61(7):. (PMID: 28438939)
      J Microbiol Methods. 2011 Sep;86(3):313-9. (PMID: 21699924)
      Antimicrob Agents Chemother. 2017 Mar 24;61(4):. (PMID: 28167556)
      Nature. 2013 May 9;497(7448):254-7. (PMID: 23584588)
      Proc Natl Acad Sci U S A. 2014 May 6;111(18):6618-23. (PMID: 24748111)
      J Infect Dis. 2017 Feb 15;215(suppl_1):S28-S36. (PMID: 28375512)
      Nat Rev Microbiol. 2016 Feb;14(2):67-76. (PMID: 26751509)
      Lancet Infect Dis. 2013 Sep;13(9):785-96. (PMID: 23969216)
      Mol Microbiol. 2019 Jun;111(6):1558-1570. (PMID: 30875129)
      BMC Bioinformatics. 2007 May 23;8:172. (PMID: 17521438)
      Sci Rep. 2016 Aug 17;6:31644. (PMID: 27531594)
      JAMA. 2013 Apr 17;309(15):1573-4. (PMID: 23592085)
      PLoS Genet. 2019 Apr 15;15(4):e1008114. (PMID: 30986243)
      Nature. 2016 Feb 25;530(7591):499-503. (PMID: 26863189)
      Genetics. 2017 Aug;206(4):1727-1738. (PMID: 28634160)
      mBio. 2014 Jun 24;5(3):e01355-14. (PMID: 24961694)
      Int J Antimicrob Agents. 2019 Aug;54(2):117-124. (PMID: 30885806)
      Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5. (PMID: 10829079)
      Mol Microbiol. 2014 Jun;92(5):1072-91. (PMID: 24720807)
      J Antimicrob Chemother. 2016 Aug;71(8):2143-6. (PMID: 27073266)
      Nature. 2013 Jul 25;499(7459):394-6. (PMID: 23887414)
      mBio. 2018 May 1;9(3):. (PMID: 29717009)
      PLoS Genet. 2013;9(9):e1003742. (PMID: 24039596)
      Mol Cell. 2012 Jun 8;46(5):595-605. (PMID: 22521689)
      Genome Res. 2004 Jun;14(6):1188-90. (PMID: 15173120)
      Clin Microbiol Rev. 2012 Oct;25(4):682-707. (PMID: 23034326)
      Nat Rev Microbiol. 2015 Nov;13(11):722-36. (PMID: 26411297)
      J Antimicrob Chemother. 2020 Apr 1;75(4):890-895. (PMID: 32003793)
      Nucleic Acids Res. 2012 Jul;40(12):5569-76. (PMID: 22402487)
      Microb Drug Resist. 2020 Jun;26(6):630-636. (PMID: 31834846)
      J Basic Microbiol. 2017 Apr;57(4):325-336. (PMID: 28156004)
      Nat Microbiol. 2019 Nov;4(11):1919-1929. (PMID: 31358985)
      mSphere. 2016 Jun 01;1(3):. (PMID: 27303749)
    • Contributed Indexing:
      Keywords: CRISPR-Cas; Klebsiella pneumoniae clonal complex 258; carbapenem resistance; horizontal gene transfer; plasmids
    • Accession Number:
      0 (Bacterial Proteins)
      EC 3.5.2.6 (beta-Lactamases)
      EC 3.5.2.6 (carbapenemase-2, Klebsiella pneumoniae)
    • Publication Date:
      Date Created: 20200513 Date Completed: 20201204 Latest Revision: 20210428
    • Publication Date:
      20240105
    • Accession Number:
      PMC7301723
    • Accession Number:
      10.1080/22221751.2020.1763209
    • Accession Number:
      32393110