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Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks ( Theobroma cacao L.) Using Celluclast ® 1.5 L.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, c1995-
    • Subject Terms:
    • Abstract:
      Cocoa pod husks are a waste generated during the processing of cocoa beans. We aimed to explore the enzymatic extraction of pectin using cellulases. The extraction process was optimized using a central composite design (CCD) and analyzed by response surface methodology (RSM). The parameters optimized were feedstock concentration (%), enzyme dosage (µL/g), and time (h). Three dependent variables were studied: pectin yield (g/100 g dry husk) (R 2 = 97.02), galacturonic acid content (g/100 g pectin) (R 2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R 2 = 95.35). The optimal parameters were 6.0% feedstock concentration, 40 µL g -1 of enzyme, and 18.54 h, conditions that produced experimentally a pectin yield of 10.20 g/100 g feedstock, 52.06 g galacturonic acid/100 g pectin, and a yield 5.31 g galacturonic acid/100 g feedstock. Using the chemical extraction method, a yield of 8.08 g pectin/100 g feedstock and a galacturonic acid content of 60.97 g/100 g pectin were obtained. Using assisted sonication, a pectin yield of 8.28 g/100 g feedstock and a galacturonic acid content of 42.77 g/100 g pectin were obtained. Enzymatically optimized pectin has rheological and physicochemical features typical of this biomaterial, which provides an interesting alternative for the valorization of cocoa husks.
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    • Grant Information:
      Convocatoria 755 de 2016 Sistema General de Regalías de Colombia; Convocatoria 755 de 2016 Departamento Administrativo de Ciencia, Tecnología e Innovación (COLCIENCIAS)
    • Contributed Indexing:
      Keywords: agricultural waste; byproducts; cellulase; optimization
    • Accession Number:
      0 (Hexuronic Acids)
      0 (Waste Products)
      4JK6RN80GF (galacturonic acid)
      89NA02M4RX (Pectins)
      EC 3.2.1.- (Cellulases)
    • Publication Date:
      Date Created: 20210403 Date Completed: 20210513 Latest Revision: 20210513
    • Publication Date:
      20240104
    • Accession Number:
      PMC7963153
    • Accession Number:
      10.3390/molecules26051473
    • Accession Number:
      33803082