Transcriptomic analyses of cacao flavonoids produced in photobioreactors.

Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE

Original Publication: London : BioMed Central, [2000-

Cacao*/genetics; Flavonoids ; Gene Expression Regulation, Plant ; Photobioreactors ; Transcriptome

Background: Theobroma cacao is a major source of flavonoids such as catechins and their monomers proanthocyanidins (PAs), widely studied for their potential benefits in cardiovascular diseases. Light has been shown to promote plant secondary metabolite production in vitro. In this study, cacao cells cultured in 7.5 L stirred tank photobioreactors (STPs) were exposed to a change of white to blue LED lights for 28 days (d).
Results: Transcriptomic analyses were performed in three time points comparing changing expression patterns, after cell exposure to white light (d0-VS-d14), after a shift from white to blue light (d14-VS-d15), and after an extended period of blue light for the following 15 days (d15-VS-d28). Under white light, there was enrichment in metabolic pathways associated with cell growth (carbon, glycolysis, and amino acid biosynthesis) accompanied by a significant increase in the PAs content. In the shift to blue light, further increase in PAs content was observed concomitantly with the significant expression of TWO-COMPONENT RESPONSE REGULATOR genes involved in the early stress responses via circadian clock and hormone pathways. Under blue light exposure, we observed a depletion of PAs content associated with ROS-mediated stress pathways.
Conclusions: Light effects on large-scale cell cultures in photobioreactors are complex and pleiotropic; however, we have been able to identify key regulatory players upstream cacao flavonoid biosynthesis in STPs, including TWO-COMPONENT SYSTEM and ROS-signaling genes. The crosstalk between flavonoid biosynthesis and regulatory networks led to understand the dynamics of flavonoid production and degradation in response to light-driven ROS signals. This can be used to optimize the time, and the yield of in vitro targeted metabolites in large-scale culture systems.
(© 2021. The Author(s).)

J Exp Bot. 2007;58(15-16):4397-406. (PMID: 18182441)
Plant Signal Behav. 2011 May;6(5):709-11. (PMID: 21448007)
Genome Biol. 2014;15(12):550. (PMID: 25516281)
Plant Cell. 2007 Mar;19(3):731-49. (PMID: 17337630)
Plant J. 2017 May;90(4):683-697. (PMID: 28008680)
Plant Physiol. 2006 Jun;141(2):436-45. (PMID: 16603662)
FEBS Lett. 2013 May 21;587(10):1543-7. (PMID: 23583450)
Nucleic Acids Res. 2017 Jan 4;45(D1):D1040-D1045. (PMID: 27924042)
Plant Mol Biol. 2005 Jan;57(2):155-71. (PMID: 15821875)
Plant Signal Behav. 2013 Oct;8(10):doi: 10.4161/psb.25761. (PMID: 23887492)
Front Plant Sci. 2019 Jan 08;9:1920. (PMID: 30671072)
New Phytol. 2005 Jan;165(1):9-28. (PMID: 15720617)
Sci Rep. 2019 Jan 24;9(1):437. (PMID: 30679715)
Mol Plant. 2016 Oct 10;9(10):1353-1365. (PMID: 27435853)
Sci Rep. 2018 Sep 11;8(1):13575. (PMID: 30206304)
Bioinformatics. 2005 Jun;21 Suppl 1:i159-68. (PMID: 15961453)
Sci Rep. 2016 Oct 20;6:35778. (PMID: 27762345)
DNA Res. 2010 Oct;17(5):303-24. (PMID: 20817745)
Science. 2001 Nov 2;294(5544):1108-11. (PMID: 11691995)
J Photochem Photobiol B. 2016 Oct;163:87-91. (PMID: 27541569)
Nucleic Acids Res. 2011 Jul;39(Web Server issue):W316-22. (PMID: 21715386)
Plant Physiol. 2005 Jun;138(2):1083-96. (PMID: 15923334)
Plant Cell Physiol. 2013 Jun;54(6):817-26. (PMID: 23612932)
Plant Physiol. 2011 Jan;155(1):93-100. (PMID: 21045124)
J Photochem Photobiol B. 2018 Jun;183:258-265. (PMID: 29747145)
Trends Plant Sci. 2017 Jan;22(1):11-19. (PMID: 27666517)
Plant Physiol. 2015 Dec;169(4):3002-20. (PMID: 26453433)
Nat Genet. 2000 May;25(1):25-9. (PMID: 10802651)
Plant Physiol. 1997 Dec;115(4):1405-1412. (PMID: 12223873)
Nat Genet. 2011 Feb;43(2):101-8. (PMID: 21186351)
BMC Plant Biol. 2013 Dec 05;13:202. (PMID: 24308601)
Structure. 2006 Dec;14(12):1791-9. (PMID: 17161369)
Plant J. 2014 Jul;79(2):285-98. (PMID: 24861705)
J Plant Physiol. 2014 Oct 15;171(16):1491-9. (PMID: 25105234)
Phys Chem Chem Phys. 2017 Sep 27;19(37):25598-25609. (PMID: 28905057)
J Exp Bot. 2014 Jun;65(11):2835-45. (PMID: 24220656)
Br J Clin Pharmacol. 2013 Mar;75(3):716-27. (PMID: 22775434)
Front Plant Sci. 2016 Dec 26;7:1950. (PMID: 28082998)
Plant Signal Behav. 2015;10(8):e1042647. (PMID: 26179959)
PLoS One. 2017 Aug 7;12(8):e0182348. (PMID: 28786984)
J Photochem Photobiol B. 2016 Jan;154:51-6. (PMID: 26688290)
Free Radic Biol Med. 2018 Jul;122:116-129. (PMID: 29567393)
Nat Plants. 2016 Nov 21;2:16182. (PMID: 27869786)
Biosci Biotechnol Biochem. 2014;78(9):1457-70. (PMID: 25209493)
Bioinformatics. 2012 Jun 15;28(12):1647-9. (PMID: 22543367)
Annu Rev Plant Biol. 2004;55:373-99. (PMID: 15377225)
Genome Biol. 2010;11(10):R106. (PMID: 20979621)
Appl Microbiol Biotechnol. 2018 Oct;102(20):8661-8675. (PMID: 30099571)
Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2428-32. (PMID: 11842215)
J Exp Bot. 2007;58(10):2595-607. (PMID: 17545225)
Sci Rep. 2017 Oct 24;7(1):13875. (PMID: 29066723)
J Plant Res. 2016 Mar;129(2):111-4. (PMID: 26860414)
Front Plant Sci. 2017 Feb 07;8:108. (PMID: 28223989)
Plant Signal Behav. 2012 Dec;7(12):1621-33. (PMID: 23072988)
Nucleic Acids Res. 1999 Jan 1;27(1):29-34. (PMID: 9847135)
Diseases. 2016 Dec 17;4(4):. (PMID: 28933419)
Plant Signal Behav. 2016 Jun 2;11(6):e1184808. (PMID: 27172458)
Plant Signal Behav. 2014;9(1):e27522. (PMID: 24393776)
Nucleic Acids Res. 2000 Jan 1;28(1):33-6. (PMID: 10592175)
Plant Cell Environ. 2010 Jan;33(1):88-103. (PMID: 19895401)
Nat Methods. 2015 Apr;12(4):357-60. (PMID: 25751142)
Plant Physiol. 2015 Sep;169(1):840-55. (PMID: 26175515)
Nutrients. 2014 Feb 21;6(2):844-80. (PMID: 24566441)
Nature. 2007 Sep 20;449(7160):356-60. (PMID: 17704763)
Plant J. 2017 Jun;90(5):856-867. (PMID: 27801967)
Plant J. 2018 Dec;96(6):1242-1254. (PMID: 30256479)
Plant Signal Behav. 2009 Aug;4(8):790-2. (PMID: 19820325)
Plant Cell Physiol. 2012 Aug;53(8):1470-80. (PMID: 22706033)
Plant Signal Behav. 2011 Nov;6(11):1720-31. (PMID: 22041989)

Project number C007 Ruta N; Sostenibilidad 2017 2018 Universidad de Antioquia

Keywords: Flavonoids; Gene expression; Oxidative stress; Photobioreactors; Theobroma cacao

0 (Flavonoids)

Date Created: 20210720 Date Completed: 20210721 Latest Revision: 20231101

20240105

PMC8287782

10.1186/s12864-021-07871-0

34281511