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Amplicon-based next-generation sequencing reveals the co-existence of multiple Leishmania species in patients with visceral leishmaniasis.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Canada NLM ID: 9610933 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-3511 (Electronic) Linking ISSN: 12019712 NLM ISO Abbreviation: Int J Infect Dis Subsets: MEDLINE
    • Publication Information:
      Publication: Hamilton, ON : Elsevier
      Original Publication: Hamilton, ON : Decker, c1996-
    • Subject Terms:
    • Abstract:
      Visceral leishmaniasis (VL) is a mammalian protozoal disease propagated in the Americas by female phlebotomine sandflies, mainly caused by Leishmania infantum. However, in recent years, cases of VL caused by different Leishmania species, such as L. amazonensis and L. colombiensis, have been reported in the continent. This study used an amplicon-based next-generation sequencing approach to identify VL aetiologic species using high-depth sequencing targeting a region on the Heat Shock Protein 70 gene. In this first approach, six samples from five patients diagnosed with VL were selected and analysed to identify DNA of Leishmania spp. All samples harboured DNA of L. infantum; five samples were found to be co-infected with other Leishmania spp. or with Trypanosoma cruzi, and just one sample was mono-infected with L. infantum. This study demonstrates the usefulness of this methodology to identify trypanosomatid co-infections in clinical samples, which presents an interesting study panorama considering their biological, clinical and epidemiological implications.
      Competing Interests: Conflict of interest statement None declared.
      (Copyright © 2021. Published by Elsevier Ltd.)
    • Contributed Indexing:
      Keywords: Amplicon-based NGS; Co-infection; HSP70; Leishmania; Trypanosomatid; Visceral leishmaniasis
    • Publication Date:
      Date Created: 20211205 Date Completed: 20220118 Latest Revision: 20220118
    • Publication Date:
      20240105
    • Accession Number:
      10.1016/j.ijid.2021.11.029
    • Accession Number:
      34863923