Resveratrol Effects on NRF1 Expression in Goat Oocytes During In Vitro Maturation and Fertilization.

تتناول هذه المقالة تأثيرات الريسفيراترول، وهو مضاد أكسدة طبيعي متعدد الفينولات، على تعبير جين عامل التنفس النووي 1 (NRF1) في بويضات الماعز خلال النضج في المختبر (IVM) والتلقيح في المختبر (IVF). يُعد عامل التنفس النووي 1 (NRF1) عامل نسخ رئيسي ينظم تكوين الميتوكوندريا والتمثيل الغذائي التأكسدي، وهما أمران حاسمان لجودة البويضة وكفاءتها التطورية. أظهرت الدراسة أن مكملات الريسفيراترول زادت بشكل ملحوظ من تعبير mRNA الخاص بـ NRF1 في مرحلتي النضج والتلقيح، مما ارتبط بتحسن معدلات نضج البويضات مقارنةً بالمجموعة الضابطة. تشير هذه النتائج إلى أن الريسفيراترول يعزز وظيفة الميتوكوندريا ويقلل من الإجهاد التأكسدي في بويضات الماعز، مما قد يحسن نتائج التلقيح في المختبر ويدعم استخدامه كمادة مضافة وظيفية في تقنيات الإنجاب المساعدة للماعز. [Extracted from the article]

In vitro fertilization (IVF) success in goats largely depends on oocyte quality and mitochondrial activity during vitro maturation (IVM) and fertilization. The nuclear respiratory factor 1 (NRF1) plays central role in regulation mitochondrial biogenesis and oxidative metabolism, which are essential for oocyte competence. Resveratrol, a natural polyphenolic compound with antioxidant and mitochondrial-activating properties, has been reported to enhance the SIRT1–PGC-1α–NRF1 signaling pathway, improving cellular energy metabolism. The aimed of present study to evaluation the impact of resveratrol supplements on NRF1 gene expression during IVF and IVM of goat’s oocytes. The ovaries in present study were collected from slaughterhouses, and cumulus oocyte complexes (COCs) were aspirated from antral follicles and cultured in TCM-199 medium either without or with resveratrol supplementation. After maturation and fertilization, NRF1 mRNA expression levels were quantified using quantitative realtime PCR (qRT-PCR) and normalize against GAPDH. The results showed that resveratrol significantly increased NRF1 expression at both stages of oocyte development. During IVM, NRF1 levels were higher in resveratrol-treated oocytes (2.87 ± 0.31) compared to control (1.57 ± 0.19). Similarly, during IVF, expression levels were markedly elevated (8.68 ± 0.62 vs. 3.42 ± 0.32, respectively; p ≤ 0.05). In conclusion, resveratrol supplementation enhances NRF1 gene expression through IVMand fertilization, suggesting a beneficial role in improves mitochondria functions, cytoplasmic maturation, and development potential of goat oocyte. [ABSTRACT FROM AUTHOR]

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