C1q Tumor Necrosis Factor alpha-related Protein Isoform 5 Is Increased in Mitochondrial DNA-depleted Myocytes and Activates AMP-activated Protein Kinase

Depletion of mtDNA in myocytes causes insulin resistance and alters nuclear gene expression that may be involved in rescuing processes against cellular stress. Here we show that the expression of C1q tumor necrosis factor a-related protein isoform 5 (C1QTNF5) is drastically increased following depletion of mtDNA in myocytes. C1QTNF5 is homologous to adiponectin in respect to domain structure, and its expression and secretion from myocytes correlated negatively with the cellular mtDNA content. Similar to adiponectin, C1QTNF5 induced the phosphorylation of AMP-activated protein kinase (AMPK), leading to increased cell surface recruitment of GLUT4 and increased glucose uptake. Treatment of cells with purified recombinant C1QTNF5 increased the phosphorylation of acetyl-CoA carboxylase and stimulated fatty acid oxidation. C1QTNF5-mediated phosphorylation of AMPK or acetyl-CoA carboxylase was unaffected by depletion of adiponectin receptors such as AdipoR1 or AdipoR2, which indicated that adiponectin receptors do not participate in C1QTNF5-induced activation of AMPK. Serum C1QTNF5 levels were significantly higher in obese/diabetic animals (OLETF rats, ob/ob mice, and db/db mice). These results highlight C1QTNF5 as a putative biomarker for mitochondrial dysfunction and a potent activator of AMPK. ; Wong GW, 2008, BIOCHEM J, V416, P161, DOI 10.1042/BJ20081240 ; Palanivel R, 2007, CARDIOVASC RES, V75, P148, DOI 10.1016/j.cardiores.2007.04.011 ; Ryan MT, 2007, ANNU REV BIOCHEM, V76, P701, DOI 10.1146/annurev.biochem.76.052305.091720 ; Yoon MJ, 2006, DIABETES, V55, P2562, DOI 10.2337/db05-1322 ; Hardie DG, 2006, J PHYSIOL-LONDON, V574, P7, DOI 10.1113/jphysiol.2006.108944 ; Akiyama H, 2006, FEBS J, V273, P2257, DOI 10.1111/j.1742-4658.2006.05240.x ; Maeda T, 2006, J CELL PHYSIOL, V206, P537, DOI 10.1002/jcp.20493 ; Lasser G, 2006, BLOOD, V107, P423, DOI 10.1182/blood-2005-04-1425 ; Fang X, 2005, J MOL ENDOCRINOL, V35, P465, DOI 10.1677/jme.1.01877 ; Biswas G, 2005, GENE, V354, P132, DOI 10.1016/j.gene.2005.03.028 ; Tang ...