Abstract: To overcome the variation of lipid productivity of C. vulgaris, the objective of the present research was to investigate the effect of various growth medium compositions on Chlorella vulgaris (C. vulgaris) in an attempt to enhance its growth and lipid production using batch culture conditions. Thirteen different growth media were being tested in culture tubes in stage 1. Maximum optical density reading was recorded for the bold basal medium (OD683: 3.389 ± 0.023). Besides, in the large scale experiment, Bold basal medium was observed the highest biomass productivity (114.208 ± 0.850 mg L-1day-1) in day 12 and specific growth rate (d-1) (0.279 ± 0.001). Highest lipid content (17.640 ± 0.002%, day 12), lipid productivity (250.576 ± 4.834 mg/L, day 12) and overall lipid productivity of 20.881 ± 0.403mg L-1day-1 was also achieved the highest in Bold basal medium, by comparing with other selected medium. Further study is required with a focus on one or two particular nutrients (with alteration in concentration) in one type of medium, and other factors remaining unchanged each time to find out the induce effect of nutrients to the biomass and lipid accumulation.
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