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Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment

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  • Additional Information
    • Contributors:
      Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR); Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO EPE)-Centre National de la Recherche Scientifique (CNRS); Unité Santé, Génétique et Microbiologie des Mollusques (SGMM); Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER); Department of Biological and Environmental Sciences Gothenburg; Göteborgs Universitet = University of Gothenburg (GU); Institut des Sciences de l'Evolution de Montpellier (UMR ISEM); Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE); Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement IRD : UR226-Centre National de la Recherche Scientifique (CNRS); Laboratoire de Physiologie des Invertébrés (LPI); Physiologie Fonctionnelle des Organismes Marins (PFOM); Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)
    • Publication Information:
      CCSD
      EDP Sciences
    • Publication Date:
      2013
    • Collection:
      Université de Montpellier: HAL
    • Abstract:
      International audience ; We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.
    • Accession Number:
      10.1051/alr/2013052
    • Online Access:
      https://hal.univ-brest.fr/hal-00946729
      https://hal.univ-brest.fr/hal-00946729v1/document
      https://hal.univ-brest.fr/hal-00946729v1/file/Morvezen2013.pdf
      https://doi.org/10.1051/alr/2013052
    • Rights:
      info:eu-repo/semantics/OpenAccess
    • Accession Number:
      edsbas.CF8BAF86