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NF-YA5/miR169a MODULE CONTROLLING NITROGEN UTILIZATION EFFICIENCY OF PLANT AND USES THEREOF

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  • Publication Date:
    March 20, 2025
  • Additional Information
    • Document Number:
      20250092410
    • Appl. No:
      18/368607
    • Application Filed:
      September 15, 2023
    • Abstract:
      A NF-YA5/miR169a module controls nitrogen utilization efficiency of plant and uses thereof. Since the NF-YA5/miR169a module enables the enhancement of the nitrogen utilization efficiency in plants, it can be advantageously used for the development of environmentally friendly and cost-effective plants that can reduce the consumption of nitrogen fertilizers while maintaining crop yields.
    • Claim:
      1. A method of controlling nitrogen utilization efficiency in a plant under a nitrogen-deficient condition, the method comprising: controlling expression and/or activity of miR169a consisting of the nucleotide sequence of SEQ ID NO: 37.
    • Claim:
      2. The method according to claim 1, wherein the method comprises the controlling of the expression comprising knocking out miR169a using a gene editing system, thereby increasing the nitrogen utilization efficiency in the plant under the nitrogen-deficient condition.
    • Claim:
      3. The method according to claim 1, wherein the method comprises the controlling of the activity comprising inhibiting activity of miR169a based on knock-out of a target gene of miR169a using a gene editing system, thereby increasing the nitrogen utilization efficiency in the plant under the nitrogen-deficient condition.
    • Claim:
      4. The method according to claim 3, wherein the target gene of miR169a is NF-YA5 (Nuclear Factor Y subunit A5).
    • Claim:
      5. A method of producing a genome-edited rice plant with enhanced nitrogen utilization efficiency under a nitrogen-deficient condition, the method comprising: introducing a guide RNA specific to a target nucleotide sequence of pre-miR169a derived from rice and an endonuclease protein to a rice plant cell to have genome editing; and regenerating a rice plant from the rice plant cell that is obtained after the genome editing.
    • Claim:
      6. The method according to claim 5, wherein the target nucleotide sequence of pre-miR169a derived from rice consists of the nucleotide sequence of SEQ ID NO: 39.
    • Claim:
      7. A method of producing a genome-edited rice plant with enhanced nitrogen utilization efficiency under a nitrogen-deficient condition, the method comprising: introducing a guide RNA specific to a target nucleotide sequence of NF-YA5 gene derived from rice and an endonuclease protein to a rice plant cell to have genome editing; and regenerating a rice plant from the rice plant cell that is obtained after the genome editing.
    • Claim:
      8. The method according to claim 7, wherein the target nucleotide sequence of NF-YA5 gene derived from rice consists of the nucleotide sequence of SEQ ID NO: 41.
    • Claim:
      9. The method according to claim 8, wherein the target nucleotide sequence of NF-YA5 gene derived from rice is a binding site for miR169a.
    • Claim:
      10. A genome-edited rice plant with enhanced nitrogen utilization efficiency under the nitrogen-deficient conditions which is produced by the method of claim 7.
    • Claim:
      11. A seed of the genome-edited rice plant of claim 10.
    • Current International Class:
      12
    • Accession Number:
      edspap.20250092410