Histopathological findings in rabbits after experimental acute exposure to the Loxosceles intermedia (brown spider) venom.

Publisher: Wiley Country of Publication: England NLM ID: 9014042 Publication Model: Print Cited Medium: Print ISSN: 0959-9673 (Print) Linking ISSN: 09599673 NLM ISO Abbreviation: Int J Exp Pathol Subsets: MEDLINE

Original Publication: Oxford, OX : Wiley, c1990-

Muscle, Skeletal/*pathology ; Skin/*pathology ; Spider Bites/*pathology ; Spider Venoms/*toxicity; Animals ; Blood Vessels/pathology ; Edema/chemically induced ; Edema/pathology ; Hemorrhage/chemically induced ; Injections, Intradermal ; Muscle, Skeletal/immunology ; Necrosis ; Neutrophil Infiltration ; Rabbits ; Skin/immunology ; Thrombosis/chemically induced ; Time Factors

Loxoscelism, the term used to describe envenomation with brown spiders, is characterized by a dermonecrotic lesion at the bite site. In the present investigation we submitted albino rabbits to an acute experimental envenomation protocol using Loxosceles intermedia (brown spider) venom, with in order to determine the pathogenesic features of the lesion induced by this spider, which is the cause of several accidents throughout the world. Rabbits received intradermal injections of the venom and were monitored over the first 4 h, and then at 12 h and 1, 2 and 5 days after envenomation. Histological specimens from 3 rabbits per time point were collected from euthanized animals and processed for histological examination by light microscopy. Major findings observed during the first 4 h were oedema, haemorrhage, degeneration of blood vessel walls, plasma exudation, thrombosis, neutrophil accumulation in and around blood vessels with an intensive diapedesis, a diffuse collection of inflammatory cells (polymorphonuclear leucocytes) in the dermis, and subcutaneous muscular oedema. Over the following hours and up to 5 days after envenomation the changes progressed to massive neutrophil infiltration (with no other leucocytes) into the dermis and even into subcutaneous muscle tissue, destruction of blood vessels, thrombosis, haemorrhage, myonecrosis, and coagulative necrosis on the 5th day.

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0 (Spider Venoms)

Date Created: 20030327 Date Completed: 20030421 Latest Revision: 20190513

20250114

PMC2517689

10.1046/j.1365-2613.2002.00241.x

12657137