Immunohıstochemıcal staınıng of cd3, cd79acy and s-100 on bursa fabrıcıus, thymus and spleen of turkeys (Meleagrıs gallapavo).

Distribution of CD3, CD79acy and S-100, immunohistochemically, in the bursa of Fabricius, thymus and spleen has not been reported in turkeys. Therefore, we determined the localisation of anti-CD3 protein antibodies for mature T-lymphocytes, anti-CD79acy antibodies for B-lymphocytes and anti-S-100 protein antibodies for follicular dentritic cells in the bursa of Fabricius, thymus and spleen, which are the lymphoid organs, in a study sample of turkeys. Triple staining method was applied to demonstrate the general structure. Moreover, in all the organs positive reactions were observed with the CD3, CD79acy and S-100 antibodies. It was also found that similar areas had a positive reaction with CD79acy and S-100 in all of the tested organs. It was remarkable that CD79acy reacted positively on Hassall's corpuscles (strongly) and the reticular cells (weakly) in the medulla of the thymus instead of the B-lymphocyte positive areas. [ABSTRACT FROM AUTHOR]