Genetic characterization of sheep pox virus strains from outbreaks in Central Russia in 2018–2019.

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Author(s): Krotova, Alena¹ (AUTHOR); Shalina, Kseniya¹ (AUTHOR); Mazloum, Ali¹ (AUTHOR); Kwon, Dmitry² (AUTHOR); Van Schalkwyk, Antoinette^3,4 (AUTHOR); Byadovskaya, Olga¹ (AUTHOR); Sprygin, Alexander¹ (AUTHOR)
Source:
Transboundary & Emerging Diseases. Nov2022, Vol. 69 Issue 6, pe3430-e3435. 6p.
Subject Terms:
*Poxviruses; *Whole genome sequencing; *Sheep; *Bluetongue virus; *DNA sequencing; *Nucleotide sequencing

Additional Information
- Subject Terms:
  Srinagar (Jammu & Kashmir, India); Russia
- Abstract:
  This study investigates the phylogenomic relatedness between sheep pox viruses (SPPVs) circulating in Central Russia in 2018–2019 with the NISKHI vaccine strain used in the country, based on their complete genome sequences. The sheep pox outbreaks occurred 1 year apart in the adjacent regions of Tula and Moscow. Full genome sequences were generated by sequencing DNA directly obtained from Trizol‐extracted scabs, using the DNBSEQ‐400 platform (MGI Tech, China). Phylogenetic analysis indicated that the SPPV isolates from Russia clusters with previously published sequences from Srinagar in the Kashmir province of India in 2000 (SPPV‐Srinagar strain) as well as SPPV A strain from Kazakhstan in 2000. The aforementioned cluster belonged to a sister clade containing the NISKHI vaccine strain, thus indicating that the recent outbreaks were not genetically linked to the widely used vaccine. [ABSTRACT FROM AUTHOR]