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Construction of consecutive deletions of the Escherichia coli chromosome.

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  • Author(s): Kato J;Kato J; Hashimoto M
  • Source:
    Molecular systems biology [Mol Syst Biol] 2007; Vol. 3, pp. 132. Date of Electronic Publication: 2007 Aug 14.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: EMBO Press Country of Publication: Germany NLM ID: 101235389 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1744-4292 (Electronic) Linking ISSN: 17444292 NLM ISO Abbreviation: Mol Syst Biol Subsets: MEDLINE
    • Publication Information:
      Publication: 2024- : Heidelberg : EMBO Press
      Original Publication: London : Nature Pub. Group, c2005-
    • Subject Terms:
      Genes, Bacterial* ; Sequence Deletion*; Chromosomes, Bacterial/*genetics ; Escherichia coli/*genetics; DNA Replication/genetics ; DNA, Bacterial/genetics ; Gene Expression Regulation, Bacterial ; Genetic Complementation Test ; Plasmids/genetics ; Replication Origin/genetics
    • Abstract:
      The minimal set of genetic information necessary and sufficient to sustain a functioning cell contains not only trans-acting genes, but also cis-acting chromosomal regions that cannot be complemented by plasmids carrying these regions. In Escherichia coli (E. coli), only one chromosomal region, the origin of replication has been identified to be cis-acting. We constructed a series of mutants with long-range deletions, and the chromosomal regions containing trans-acting essential genes were deleted in the presence of plasmids complementing the deleted genes. The deleted regions cover all regions of the chromosome except for the origin and terminus of replication. The terminus affects cell growth, but is not essential. Our results indicate that the origin of DNA replication is the only vital, unique cis-acting DNA sequence in the E. coli chromosome necessary for survival.
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    • Accession Number:
      0 (DNA, Bacterial)
    • Publication Date:
      Date Created: 20070819 Date Completed: 20070914 Latest Revision: 20240104
    • Publication Date:
      20250114
    • Accession Number:
      PMC1964801
    • Accession Number:
      10.1038/msb4100174
    • Accession Number:
      17700540