Effect of Seasons on the Developmental Competency of Buffalo Oocytes.

المقال يركز على تأثير التغيرات الموسمية على كفاءة تطوير بويضات الجاموس، حيث يتم فحص معدلات الشفط، وإنتاج البويضات، والجودة، ومعدلات النضوج، وتطور الأجنة. الدراسة التي أجريت في عام 2022، قامت بتحليل 627 مبيضًا من الجاموس تم جمعها على مدار السنة، وكشفت أن الفصول الباردة (من أكتوبر إلى مارس) حسنت بشكل كبير معدلات شفط البويضات وجودتها مقارنة بالفصول الحارة (من أبريل إلى سبتمبر). أظهرت النتائج أن أعدادًا أكبر من البويضات ذات الجودة الجيدة وتطورًا أفضل للأجنة لوحظت خلال الأشهر الباردة، مما يشير إلى أن الإجهاد الحراري خلال الفصول الأكثر حرارة يؤثر سلبًا على أداء التكاثر لدى الجاموس. تسلط النتائج الضوء على أهمية أخذ التأثيرات الموسمية في الاعتبار في تربية الجاموس وإدارة التكاثر. [Extracted from the article]

The objective of this study was to determine the seasonal efficacy on the (1) aspiration rate, oocyte yield, and oocyte quality of buffalo. 2) Oocyte maturation rates and embryonic developmental competency in buffalo species. In 2022, the ovaries of buffaloes [n= 627] were collected throughout the year, including hot and moderately hot seasons (April-September) and cold seasons (October-March). The temperature humidity index (THI) was computed, the numbers of ovarian follicles and oocytes were counted, and the aspiration rate and the yield of oocytes were recorded. The quality of the oocytes was categorized as high quality (good and excellent) and low quality (fair and denuded) oocytes. Good and excellent oocytes were in vitro matured in tissue culture media-199 enriched with 10 μg/ml follicle-stimulating hormone (FSH) + 10% fetal calf serum (FCS) + 50 μg/ml gentamicin. After 22 hours of incubation with 5% CO2 at 38.5°C, the cytoplasmic and nuclear maturation of the matured oocytes was assessed. Mature oocytes were fertilized using frozen-thawed semen for eighteen hours and then cultured for seven days using modified synthetic oviductal fluid (mSOF). Our investigation showed that, in comparison to hot seasons, the aspiration rates and oocyte yield during the cold seasons were markedly (P˂0.05) higher. During cold seasons, both the number and proportion of good and excellent oocytes increased. Additionally, the expansion of cumulus cells of COCs and nuclear maturation of mature buffalo oocytes in vitro was significantly (P<0.05) decreased during the hot seasons. The morula and blastocyst rates were significantly (P˂0.05) greater in autumn and winter than in spring and summer seasons. In conclusion, reproduction in buffalo in the hot seasons could be impaired by reducing the oocyte recovery, yield, and quality. In Buffalo, the rates of in vitro maturation, morula and blastocyst increased significantly during the cold seasons. [ABSTRACT FROM AUTHOR]

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