Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

UNE-EN ISO/IEC 17025:2005-accredited method for the determination of pesticide residues in fruit and vegetable samples by LC-MS/MS.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Informa Healthcare Country of Publication: England NLM ID: 101485040 Publication Model: Print Cited Medium: Internet ISSN: 1944-0057 (Electronic) Linking ISSN: 19440057 NLM ISO Abbreviation: Food Addit Contam Part A Chem Anal Control Expo Risk Assess Subsets: MEDLINE
    • Publication Information:
      Publication: London : Informa Healthcare
      Original Publication: Abingdon, Oxon ; Philadelphia, PA : Taylor & Francis, 2008-
    • Subject Terms:
      Food Contamination*; Food Inspection/*methods ; Food Inspection/*standards ; Fruit/*chemistry ; Pesticide Residues/*analysis ; Vegetables/*chemistry; Analytic Sample Preparation Methods ; Calibration ; Chromatography, High Pressure Liquid ; Europe ; Limit of Detection ; Pesticide Residues/chemistry ; Reproducibility of Results ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry
    • Abstract:
      A rapid, simple and sensitive multi-residue method was developed and validated for the simultaneous quantification and confirmation of 69 pesticides in fruit and vegetables using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The samples were extracted following the quick, easy, cheap, effective, rugged and safe method known as QuEChERS. Mass spectrometric conditions were individually optimised for each analyte in order to achieve maximum sensitivity in multiple reaction monitoring (MRM) mode. Using the developed chromatographic conditions, 69 pesticides can be separated in less than 17 min. Two selected reaction monitoring (SRM) assays were used for each pesticide to obtain simultaneous quantification and identification in one run. With this method in SRM mode, more than 150 pesticides can be analysed and quantified, but their confirmation is not possible in all cases according to the European regulations on pesticide residues. Nine common representative matrices (zucchini, melon, cucumber, watermelon, tomato, garlic, eggplant, lettuce and pepper) were selected to investigate the effect of different matrices on recovery and precision. Mean recoveries ranged from 70% to 120%, with relative standard deviations (RSDs) lower than 20% for all the pesticides. The proposed method was applied to the analysis of more than 2000 vegetable samples from the extensive greenhouse cultivation in the province of Almeria, Spain, during one year. The methodology combines the advantages of both QuEChERS and LC-MS/MS producing a very rapid, sensitive, accurate and reliable procedure that can be applied in routine analytical laboratories. The method was validated and accredited according to UNE-EN-ISO/IEC 17025:2005 international standard (accreditation number 278/LE1027).
    • Accession Number:
      0 (Pesticide Residues)
    • Publication Date:
      Date Created: 20100824 Date Completed: 20110127 Latest Revision: 20191210
    • Publication Date:
      20250114
    • Accession Number:
      10.1080/19440049.2010.506602
    • Accession Number:
      20730643