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The synaptoneurosome transcriptome: a model for profiling the emolecular effects of alcohol.

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  • Additional Information
    • Source:
      Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101083949 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1473-1150 (Electronic) Linking ISSN: 1470269X NLM ISO Abbreviation: Pharmacogenomics J Subsets: MEDLINE
    • Publication Information:
      Original Publication: Avenet, NJ : Nature Pub. Group, c2001-
    • Subject Terms:
      Alcohol Drinking/*genetics ; Ethanol/*adverse effects ; Gene Expression/*drug effects ; Gene Expression/*genetics ; Gene Regulatory Networks/*drug effects ; Gene Regulatory Networks/*genetics ; Transcriptome/*genetics; Alcoholism/genetics ; Alcoholism/metabolism ; Amygdala/drug effects ; Amygdala/metabolism ; Animals ; Female ; Gene Expression Profiling/methods ; Humans ; Mice ; Mice, Inbred C57BL ; RNA, Messenger/genetics ; Signal Transduction/drug effects ; Signal Transduction/genetics ; Transcriptome/drug effects ; gamma-Aminobutyric Acid/metabolism
    • Abstract:
      Chronic alcohol consumption changes gene expression, likely causing persistent remodeling of synaptic structures via altered translation of mRNAs within synaptic compartments of the cell. We profiled the transcriptome from synaptoneurosomes (SNs) and paired total homogenates (THs) from mouse amygdala following chronic voluntary alcohol consumption. In SN, both the number of alcohol-responsive mRNAs and the magnitude of fold-change were greater than in THs, including many GABA-related mRNAs upregulated in SNs. Furthermore, SN gene co-expression analysis revealed a highly connected network, demonstrating coordinated patterns of gene expression and highlighting alcohol-responsive biological pathways, such as long-term potentiation, long-term depression, glutamate signaling, RNA processing and upregulation of alcohol-responsive genes within neuroimmune modules. Alterations in these pathways have also been observed in the amygdala of human alcoholics. SNs offer an ideal model for detecting intricate networks of coordinated synaptic gene expression and may provide a unique system for investigating therapeutic targets for the treatment of alcoholism.
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    • Grant Information:
      F31 AA022557 United States AA NIAAA NIH HHS; T32 AA007471 United States AA NIAAA NIH HHS; 1F31-AA022557-01 United States AA NIAAA NIH HHS; AA020683 United States AA NIAAA NIH HHS; RC2AA019382 United States AA NIAAA NIH HHS; RC2 AA019382 United States AA NIAAA NIH HHS; AA-UO1-13520 United States AA NIAAA NIH HHS; P01 AA020683 United States AA NIAAA NIH HHS; U01 AA020926 United States AA NIAAA NIH HHS; R01 AA012404 United States AA NIAAA NIH HHS; AA012404 United States AA NIAAA NIH HHS; U01 AA013520 United States AA NIAAA NIH HHS
    • Accession Number:
      0 (RNA, Messenger)
      3K9958V90M (Ethanol)
      56-12-2 (gamma-Aminobutyric Acid)
    • Publication Date:
      Date Created: 20140820 Date Completed: 20151209 Latest Revision: 20211021
    • Publication Date:
      20250114
    • Accession Number:
      PMC4334750
    • Accession Number:
      10.1038/tpj.2014.43
    • Accession Number:
      25135349