Involvement of microRNA miR-2b-3p in regulation of metabolic resistance to insecticides in Plutella xylostella.

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Author(s): Etebari K;Etebari K; Afrad MH; Afrad MH; Tang B; Tang B; Tang B; Silva R; Silva R; Furlong MJ; Furlong MJ; Asgari S; Asgari S
Source:
Insect molecular biology [Insect Mol Biol] 2018 Aug; Vol. 27 (4), pp. 478-491. Date of Electronic Publication: 2018 Mar 24.
Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
Language:
English

Additional Information
- Source:
  Publisher: Blackwell Scientific For The Royal Entomological Society Country of Publication: England NLM ID: 9303579 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2583 (Electronic) Linking ISSN: 09621075 NLM ISO Abbreviation: Insect Mol Biol Subsets: MEDLINE
- Publication Information:
  Publication: Oxford : Blackwell Scientific For The Royal Entomological Society
  Original Publication: Oxford : Published for the Royal Entomological Society by Blackwell Scientific Publications, c1992-
- Subject Terms:
  Insecticide Resistance/*genetics ; Insecticides/*pharmacology ; MicroRNAs/*genetics ; Moths/*drug effects ; Moths/*genetics; Animals ; Larva/drug effects ; Larva/genetics ; Larva/growth & development ; Larva/metabolism ; MicroRNAs/metabolism ; Moths/growth & development ; Moths/metabolism
- Abstract:
  The diamondback moth, Plutella xylostella, has developed extremely high levels of resistance to chlorantraniliprole and other classes of insecticides in the field. As microRNAs (miRNAs) play important roles in various biological processes through gene regulation, we examined the miRNA profile of P. xylostella in response to chlorantraniliprole exposure. RNA sequencing analysis showed that insecticide treatment caused significant changes in the abundance of some miRNAs. Increasing exposure time and insecticide concentration induced more dysregulated miRNAs in P. xylostella larvae. We also screened potential target genes for some of the differentially expressed miRNAs (such as miR-2b-3p, miR-14b-5p and let-7-5p), which may play important roles in insecticide resistance development. Exposure of P. xylostella larvae to chlorantraniliprole caused considerable overexpression in the transcript levels of potential target genes cytochrome P450 9f2 (CYP9F2) and 307a1 (CYP307a1). Application of miR-2b-3p and miR-14b-5p mimics significantly suppressed the relative transcript levels of CYP9F2 and CYP307a1, respectively, in a P. xylostella cell line. Furthermore, enrichment of P. xylostella diet with miR-2b-3p mimics significantly increased mortality in deltamethrin-resistant larvae when exposed to deltamethrin. The results suggest that miR-2b-3p may suppress CYP9F2 transcript levels in P. xylostella and consequently inhibit larval detoxification pathways. The findings provide an insight into possible role of miRNAs in regulation of metabolic resistance of insects to insecticides.
  (© 2018 The Royal Entomological Society.)
- Contributed Indexing:
  Keywords: Plutella xylostella; cytochrome P450; insecticide; microRNA; resistance
- Accession Number:
  0 (Insecticides)
  0 (MicroRNAs)
- Publication Date:
  Date Created: 20180325 Date Completed: 20190114 Latest Revision: 20190114
- Publication Date:
  20240513
- Accession Number:
  10.1111/imb.12387
- Accession Number:
  29573306

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