Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • Subject Terms:
      Cell Line, Tumor* ; Neoplasm Transplantation*; Brain Neoplasms/*pathology ; Glioblastoma/*pathology; Aged ; Aged, 80 and over ; Animals ; Female ; Humans ; Male ; Mice, Inbred NOD ; Mice, SCID ; Middle Aged
    • Abstract:
      Low-passage, serum-free cell lines cultured from patient tumour tissue are the gold-standard for preclinical studies and cellular investigations of glioblastoma (GBM) biology, yet entrenched, poorly-representative cell line models are still widely used, compromising the significance of much GBM research. We submit that greater adoption of these critical resources will be promoted by the provision of a suitably-sized, meaningfully-described reference collection along with appropriate tools for working with them. Consequently, we present a curated panel of 12 readily-usable, genetically-diverse, tumourigenic, patient-derived, low-passage, serum-free cell lines representing the spectrum of molecular subtypes of IDH-wildtype GBM along with their detailed phenotypic characterisation plus a bespoke set of lentiviral plasmids for bioluminescent/fluorescent labelling, gene expression and CRISPR/Cas9-mediated gene inactivation. The cell lines and all accompanying data are readily-accessible via a single website, Q-Cell (qimrberghofer.edu.au/q-cell/) and all plasmids are available from Addgene. These resources should prove valuable to investigators seeking readily-usable, well-characterised, clinically-relevant, gold-standard models of GBM.
    • Comments:
      Erratum in: Sci Rep. 2020 Jan 21;10(1):1185. doi: 10.1038/s41598-020-57850-w. (PMID: 31965010)
    • References:
      Nature. 2012 Aug 23;488(7412):522-6. (PMID: 22854781)
      Methods Mol Biol. 2011;791:33-53. (PMID: 21913070)
      J Biol Chem. 2013 Dec 27;288(52):37355-64. (PMID: 24265321)
      Acta Neuropathol. 2007 Aug;114(2):97-109. (PMID: 17618441)
      Science. 2014 Jun 20;344(6190):1396-401. (PMID: 24925914)
      Mol Imaging. 2015;14:385-99. (PMID: 26218510)
      Nat Genet. 2003 Jul;34(3):267-73. (PMID: 12808457)
      Nat Methods. 2014 Aug;11(8):783-784. (PMID: 25075903)
      Nat Biotechnol. 2011 Jan;29(1):24-6. (PMID: 21221095)
      Cancer Immunol Immunother. 2017 Sep;66(9):1217-1228. (PMID: 28501939)
      Nature. 2006 Dec 7;444(7120):756-60. (PMID: 17051156)
      EBioMedicine. 2015 Aug 15;2(10):1351-63. (PMID: 26629530)
      Oncotarget. 2017 Mar 7;8(10):16605-16620. (PMID: 28178667)
      J Med Chem. 2017 Mar 9;60(5):2052-2070. (PMID: 28206758)
      Mol Oncol. 2014 Dec;8(8):1603-15. (PMID: 25017126)
      J Neurosci Methods. 2009 Jan 30;176(2):192-9. (PMID: 19215724)
      Sci Rep. 2016 Mar 21;6:23353. (PMID: 26996336)
      Cancer Cell. 2006 May;9(5):391-403. (PMID: 16697959)
      Cancer Cell. 2010 Jan 19;17(1):98-110. (PMID: 20129251)
      Oncogenesis. 2017 May 15;6(5):e336. (PMID: 28504693)
      Biochem Pharmacol. 2015 Dec 15;98(4):587-601. (PMID: 26519552)
      PLoS One. 2011;6(12):e28674. (PMID: 22174863)
      Cancer Cell. 2017 Jul 10;32(1):42-56.e6. (PMID: 28697342)
      Nat Rev Cancer. 2014 Feb;14(2):92-107. (PMID: 24457416)
      Tumour Biol. 2014 Feb;35(2):1459-65. (PMID: 24068568)
      Cancer Cell. 2013 Feb 11;23(2):238-48. (PMID: 23410976)
      Glia. 2011 Dec;59(12):1974-86. (PMID: 21901757)
      Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):16-22. (PMID: 14603031)
      Br J Cancer. 2016 Jan 19;114(2):188-98. (PMID: 26657652)
      Cancer Cell. 2012 Dec 11;22(6):765-80. (PMID: 23238013)
      Proc Natl Acad Sci U S A. 2015 Jan 20;112(3):851-6. (PMID: 25561528)
      ACS Med Chem Lett. 2017 Mar 15;8(4):395-400. (PMID: 28435525)
      Cell Death Differ. 2008 Jan;15(1):213-5; author reply 215-6. (PMID: 17992194)
      PLoS One. 2009 Nov 13;4(11):e7752. (PMID: 19915670)
      Cell Stem Cell. 2009 Jun 5;4(6):568-80. (PMID: 19497285)
      Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):4009-14. (PMID: 23412337)
      Cancer Cell. 2013 Sep 9;24(3):331-46. (PMID: 23993863)
      Cancer Discov. 2012 May;2(5):401-4. (PMID: 22588877)
      Cell. 2013 Oct 10;155(2):462-77. (PMID: 24120142)
      JAMA. 2013 Nov 6;310(17):1842-50. (PMID: 24193082)
      Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50. (PMID: 16199517)
      Cancers (Basel). 2013 Apr 03;5(2):357-71. (PMID: 24216981)
      J Med Radiat Sci. 2015 Jun;62(2):92-8. (PMID: 26229673)
      Nucleic Acids Res. 2017 Jan 4;45(D1):D777-D783. (PMID: 27899578)
      Curr Protoc Cell Biol. 2015 Jun 01;67:23.10.1-23.10.10. (PMID: 26061242)
      J Neurooncol. 2015 Apr;122(2):263-71. (PMID: 25648357)
      Bioinformatics. 2015 May 15;31(10):1536-43. (PMID: 25583119)
      Sci Signal. 2013 Apr 02;6(269):pl1. (PMID: 23550210)
      Cancer Cell. 2006 Mar;9(3):157-73. (PMID: 16530701)
      Nat Neurosci. 2013 Oct;16(10):1373-82. (PMID: 23995067)
      Brief Bioinform. 2013 Mar;14(2):178-92. (PMID: 22517427)
      N Engl J Med. 2005 Mar 10;352(10):987-96. (PMID: 15758009)
      Genes Dev. 2015 Jun 15;29(12):1203-17. (PMID: 26109046)
      Oncotarget. 2016 May 17;7(20):29306-20. (PMID: 27083054)
    • Publication Date:
      Date Created: 20190322 Date Completed: 20201015 Latest Revision: 20210109
    • Publication Date:
      20250114
    • Accession Number:
      PMC6427001
    • Accession Number:
      10.1038/s41598-019-41277-z
    • Accession Number:
      30894629