Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Radiation effects on early phase of NT2/D1 neural differentiation in vitro.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Taylor & Francis Country of Publication: England NLM ID: 8809243 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1362-3095 (Electronic) Linking ISSN: 09553002 NLM ISO Abbreviation: Int J Radiat Biol Subsets: MEDLINE
    • Publication Information:
      Original Publication: London ; New York : Taylor & Francis, [c1988-
    • Subject Terms:
      Cell Differentiation/*radiation effects ; Neural Stem Cells/*cytology ; Neural Stem Cells/*radiation effects; Apoptosis/radiation effects ; Biomarkers/metabolism ; Cell Count ; Cellular Senescence/radiation effects ; Dose-Response Relationship, Radiation ; Gene Expression Regulation/radiation effects ; Humans ; MicroRNAs/genetics ; Neural Stem Cells/metabolism ; Time Factors
    • Abstract:
      Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of γ radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes ( SOX2 , OCT4 , SOX3 , PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.
    • Contributed Indexing:
      Keywords: NT2/D1 cell line; markers of pluripotency; miRNAs; neural differentiation; radiation
    • Accession Number:
      0 (Biomarkers)
      0 (MicroRNAs)
    • Publication Date:
      Date Created: 20190912 Date Completed: 20200123 Latest Revision: 20200123
    • Publication Date:
      20250114
    • Accession Number:
      10.1080/09553002.2019.1665207
    • Accession Number:
      31509479