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Comparison between enzyme-linked immunosorbent assay and indirect immunofluorescence for detection of antineutrophil cytoplasmic antibodies.

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  • Additional Information
    • Source:
      Publisher: Instituto de Ensino e Pesquisa Albert Einstein Country of Publication: Brazil NLM ID: 101281800 Publication Model: eCollection Cited Medium: Internet ISSN: 2317-6385 (Electronic) Linking ISSN: 16794508 NLM ISO Abbreviation: Einstein (Sao Paulo) Subsets: MEDLINE
    • Publication Information:
      Original Publication: São Paulo, SP : Instituto de Ensino e Pesquisa Albert Einstein
    • Subject Terms:
      Antibodies, Antineutrophil Cytoplasmic/*blood ; Enzyme-Linked Immunosorbent Assay/*methods ; Fluorescent Antibody Technique, Indirect/*methods; Autoimmune Diseases/blood ; Autoimmune Diseases/diagnosis ; Autoimmune Diseases/immunology ; Humans ; Predictive Value of Tests ; Reference Standards ; Reference Values ; Reproducibility of Results
    • Abstract:
      Objective: To evaluate the performance of enzyme-linked immunosorbent assay and indirect immunofluorescence methods for the detection of antineutrophil cytoplasmic antibodies in a routine clinical laboratory setting.
      Methods: A total of 227 samples were tested by indirect immunofluorescence and enzyme-linked immunosorbent assay with antigen specificity for antiproteinase 3 and antimyeloperoxidase. The proportions of positive samples were compared by McNemar hypotheses and agreement was described by Cohen's Kappa coefficient.
      Results: The agreement of the tests was 96.5%, and the Kappa coefficient obtained was 0.70 (95%CI: 0.50-0.90; p<0.001). Considering indirect immunofluorescence as the gold standard, the sensitivity of the enzyme-linked immunosorbent assay was 0.62 and the specificity was 0.99, with diagnostic accuracy in 96% of cases. Some samples were negative in enzyme-linked immunosorbent assay and positive in indirect immunofluorescence. This situation occurred in all immunofluorescence patterns, but particularly in atypical patterns. Two samples with antiproteinase 3 positivity were considered negative in indirect immunofluorescence.
      Conclusion: The enzyme-linked immunosorbent assay had high specificity but lower sensitivity. The performance of indirect immunofluorescence increases diagnostic sensitivity, while the search for antiproteinase 3 by enzyme-linked immunosorbent assay may also add diagnostic power.
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    • Accession Number:
      0 (Antibodies, Antineutrophil Cytoplasmic)
    • Publication Date:
      Date Created: 20200130 Date Completed: 20200323 Latest Revision: 20200323
    • Publication Date:
      20250114
    • Accession Number:
      PMC6980292
    • Accession Number:
      10.31744/einstein_journal/2020AO5132
    • Accession Number:
      31994608