Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

CCAAT-Binding Factor/*metabolism ; Fungal Proteins/*metabolism ; Fusarium/*metabolism ; Transcription Factors/*metabolism; CCAAT-Binding Factor/genetics ; Cell Nucleus/genetics ; DNA Damage/genetics ; Fungal Proteins/genetics ; Fusarium/genetics ; Gene Expression Regulation, Fungal ; Mycotoxins ; Transcription Factors/genetics ; Virulence

The CCAAT sequence is a ubiquitous cis-element of eukaryotic promoters, and genes containing CCAAT sequences have been shown to be activated by the CCAAT-binding transcription factor complex in several eukaryotic model organisms. In general, CCAAT-binding transcription factors form heterodimers or heterotrimeric complexes that bind to CCAAT sequences within the promoters of target genes and regulate various cellular processes. To date, except Hap complex, CCAAT-binding complex has been rarely reported in fungi. In this study, we characterized two CCAAT-binding transcription factors (Fct1 and Fct2) in the plant pathogenic fungus Fusarium graminearum. Previously, FCT1 and FCT2 were shown to be related to DNA damage response among eight CCAAT-binding transcription factors in F. graminearum. We demonstrate that the nuclear CCAAT-binding complex of F. graminearum has important functions in various fungal developmental processes, not just DNA damage response but virulence and mycotoxin production. Moreover, the results of biochemical and genetic analyses revealed that Fct1 and Fct2 may form a complex and play distinct roles among the eight CCAAT-binding transcription factors encoded by F. graminearum. To the best of our knowledge, the results of this study represent a substantial advancement in our understanding of the molecular mechanisms underlying the functions of CCAAT-binding factors in eukaryotes.

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0 (CCAAT-Binding Factor)
0 (Fungal Proteins)
0 (Mycotoxins)
0 (Transcription Factors)

Date Created: 20200319 Date Completed: 20201116 Latest Revision: 20240328

20250114

PMC7078317

10.1038/s41598-020-61885-4

32184445