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Denaturing gradient gel electrophoresis and multi-SIR profiles of soil microbial communities from a karst doline at Aggtelek National Park, Hungary.

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  • Additional Information
    • Source:
      Publisher: Springer Country of Publication: United States NLM ID: 0376757 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1874-9356 (Electronic) Linking ISSN: 00155632 NLM ISO Abbreviation: Folia Microbiol (Praha) Subsets: MEDLINE
    • Publication Information:
      Publication: New York : Springer
      Original Publication: Praha, Academia Scientiarum Bohemoslovenica.
    • Subject Terms:
      Parks, Recreational* ; Soil Microbiology*; Microbiota/*physiology; Bacteria/classification ; Bacteria/genetics ; Bacteria/metabolism ; Carbon/metabolism ; Cluster Analysis ; Denaturing Gradient Gel Electrophoresis ; Geological Phenomena ; Hungary ; RNA, Ribosomal, 16S/genetics ; Seasons ; Soil/chemistry
    • Abstract:
      Soils play an important role in the ecosystem of karstic landscapes both as a buffer zone and as a source of acidity to belowground water. Although the microbiota of karstic soils is known to have a great effect on karstification processes, the activity and composition of these communities are largely unknown. This study gives a comparative analysis of soil microbial profiles from different parts of a doline located at Aggtelek, Hungary. The aim was to reveal the relationships between the vegetation type and genetic fingerprints and substrate utilisation (multi-SIR) profiles of the soil microbiota. Soil samples were collected in early and late springs along a transect in a doline covered with different types of vegetation. Genetic fingerprints of bacterial communities were examined by denaturing gradient gel electrophoresis (DGGE) based on the 16S rRNA gene, along with multi-SIR profiles of the microbial communities measured by the MicroResp method using 15 different carbon sources. Genetic fingerprinting indicated that vegetation cover had a strong effect on the composition of soil bacterial communities. Procrustean analysis showed only a weak connection between DGGE and multi-SIR profiles, probably due to the high functional redundancy of the communities. Seasonality had a significant effect on substrate usage, which can be an important factor to consider in future studies.
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    • Grant Information:
      K79135 OTKA
    • Contributed Indexing:
      Keywords: Bacteria; DGGE; Karst soil; MicroResp; Multi-SIR
    • Accession Number:
      0 (RNA, Ribosomal, 16S)
      0 (Soil)
      7440-44-0 (Carbon)
    • Publication Date:
      Date Created: 20201008 Date Completed: 20210729 Latest Revision: 20210729
    • Publication Date:
      20250114
    • Accession Number:
      PMC7854432
    • Accession Number:
      10.1007/s12223-020-00828-y
    • Accession Number:
      33030669