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Etiology and clinical characteristics of SARS-CoV-2 and other human coronaviruses among children in Zhejiang Province, China 2017-2019.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101231645 Publication Model: Electronic Cited Medium: Internet ISSN: 1743-422X (Electronic) Linking ISSN: 1743422X NLM ISO Abbreviation: Virol J Subsets: MEDLINE
    • Publication Information:
      Original Publication: [London] : BioMed Central, 2004-
    • Subject Terms:
      COVID-19/*diagnosis ; Multiplex Polymerase Chain Reaction/*methods ; Respiratory Tract Infections/*virology ; SARS-CoV-2/*genetics; Adolescent ; Betacoronavirus/genetics ; Betacoronavirus/isolation & purification ; COVID-19/complications ; COVID-19/genetics ; COVID-19/physiopathology ; Child ; Child, Preschool ; China/epidemiology ; Coronavirus/genetics ; Coronavirus/isolation & purification ; Coronavirus 229E, Human/genetics ; Coronavirus 229E, Human/isolation & purification ; Coronavirus NL63, Human/genetics ; Coronavirus NL63, Human/isolation & purification ; Coronavirus OC43, Human/genetics ; Coronavirus OC43, Human/isolation & purification ; Female ; Hospitalization ; Humans ; Infant ; Infant, Newborn ; Male ; Phylogeny ; Respiratory Tract Infections/complications ; Respiratory Tract Infections/etiology ; SARS-CoV-2/isolation & purification ; Spike Glycoprotein, Coronavirus/genetics
    • Abstract:
      Background: A novel coronavirus (SARS-CoV-2) emerging has put global public health institutes on high alert. Little is known about the epidemiology and clinical characteristics of human coronaviruses infections in relation to infections with other respiratory viruses.
      Methods: From February 2017 to December 2019, 3660 respiratory samples submitted to Zhejiang Children Hospital with acute respiratory symptoms were tested for four human coronaviruses RNA by a novel two-tube multiplex reverse transcription polymerase chain reaction assays. Samples were also screened for the occurrence of SARS-CoV-2 by reverse transcription-PCR analysis.
      Results: Coronavirus RNAs were detected in 144 (3.93%) specimens: HCoV-HKU1 in 38 specimens, HCoV-NL63 in 62 specimens, HCoV-OC43 in 38 specimens and HCoV-229E in 8 specimens. Genomes for SARS-CoV-2 were absent in all specimens by RT-PCR analysis during the study period. The majority of HCoV infections occurred during fall months. No significant differences in gender, sample type, year were seen across species. 37.5 to 52.6% of coronaviruses detected were in specimens testing positive for other respiratory viruses. Phylogenic analysis identified that Zhejiang coronaviruses belong to multiple lineages of the coronaviruses circulating in other countries and areas.
      Conclusion: Common HCoVs may have annual peaks of circulation in fall months in the Zhejiang province, China. Genetic relatedness to the coronaviruses in other regions suggests further surveillance on human coronaviruses in clinical samples are clearly needed to understand their patterns of activity and role in the emergence of novel coronaviruses.
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    • Grant Information:
      2017ZX10103008-002 National Major Science and Technology Projects of China; CX-9 Health Leading Talents Program of Zhejiang; 2020T130104ZX China Postdoctoral Science Foundation; ZJ2020027 Zhejiang Provincial Postdoctoral Science Foundation (CN)
    • Contributed Indexing:
      Keywords: Acute respiratory infection; Hospitalized children; Human coronavirus; Phylogenic analysis; SARS-CoV-2
    • Accession Number:
      0 (Spike Glycoprotein, Coronavirus)
    • Publication Date:
      Date Created: 20210501 Date Completed: 20210514 Latest Revision: 20210514
    • Publication Date:
      20231215
    • Accession Number:
      PMC8085659
    • Accession Number:
      10.1186/s12985-021-01562-8
    • Accession Number:
      33931105