Control over Multiple Nano- and Secondary Structures in Peptide Self-Assembly.

Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 0370543 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-3773 (Electronic) Linking ISSN: 14337851 NLM ISO Abbreviation: Angew Chem Int Ed Engl

Publication: <2004-> : Weinheim : Wiley-VCH
Original Publication: Weinheim/Bergstr. : New York, : Verlag Chemie ; Academic Press, c1962-

Peptides*/chemistry ; Protein Structure, Secondary*; Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Nanostructures/chemistry ; Hydrogen-Ion Concentration ; Nanofibers/chemistry ; Humans ; Microbial Sensitivity Tests

Herein, we report the rich morphological and conformational versatility of a biologically active peptide (PEP-1), which follows diverse self-assembly pathways to form up to six distinct nanostructures and up to four different secondary structures through subtle modulation in pH, concentration and temperature. PEP-1 forms twisted β-sheet secondary structures and nanofibers at pH 7.4, which transform into fractal-like structures with strong β-sheet conformations at pH 13.0 or short disorganized elliptical aggregates at pH 5.5. Upon dilution at pH 7.4, the nanofibers with twisted β-sheet secondary structural elements convert into nanoparticles with random coil conformations. Interestingly, these two self-assembled states at pH 7.4 and room temperature are kinetically controlled and undergo a further transformation into thermodynamically stable states upon thermal annealing: whereas the twisted β-sheet structures and corresponding nanofibers transform into 2D sheets with well-defined β-sheet domains, the nanoparticles with random coil structures convert into short nanorods with α-helix conformations. Notably, PEP-1 also showed high biocompatibility, low hemolytic activity and marked antibacterial activity, rendering our system a promising candidate for multiple bio-applications.
(© 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.)

Chem Commun (Camb). 2019 Dec 7;55(94):14119-14122. (PMID: 31687686)
Angew Chem Int Ed Engl. 2014 Jul 1;53(27):6866-81. (PMID: 24920517)
J Am Chem Soc. 2001 Sep 26;123(38):9235-8. (PMID: 11562202)
J Pept Sci. 2013 Dec;19(12):737-44. (PMID: 24123618)
Biomacromolecules. 2018 Jun 11;19(6):1888-1917. (PMID: 29718664)
Angew Chem Int Ed Engl. 2018 Jun 25;57(26):7709-7713. (PMID: 29603545)
Biochim Biophys Acta. 2009 Sep;1788(9):1722-30. (PMID: 19482005)
Angew Chem Int Ed Engl. 2001 Jul 2;40(13):2382-2426. (PMID: 11443654)
Proc Natl Acad Sci U S A. 1986 Nov;83(21):8069-72. (PMID: 3464944)
Angew Chem Int Ed Engl. 2018 Feb 5;57(6):1537-1542. (PMID: 29266653)
Acc Chem Res. 2017 Oct 17;50(10):2440-2448. (PMID: 28876055)
J Am Chem Soc. 2010 May 5;132(17):6041-6. (PMID: 20377229)
Langmuir. 2018 Jul 10;34(27):8065-8074. (PMID: 29897242)
Chem Sci. 2019 Aug 20;10(40):9358-9366. (PMID: 32110300)
Chem Rev. 2016 Feb 24;116(4):2414-77. (PMID: 26727633)
Biophys J. 1997 Dec;73(6):2960-4. (PMID: 9414210)
Org Biomol Chem. 2021 Mar 28;19(12):2804-2810. (PMID: 33720265)
Chem Commun (Camb). 2020 Jun 23;56(50):6757-6769. (PMID: 32462151)
FEBS Lett. 2015 Dec 21;589(24 Pt B):3915-20. (PMID: 26555191)
Angew Chem Int Ed Engl. 2019 Nov 18;58(47):16730-16740. (PMID: 31271244)
Chem Sci. 2021 Jul 22;12(33):11197-11203. (PMID: 34522317)
Chem Soc Rev. 2021 Apr 26;50(8):5165-5200. (PMID: 33646219)
Chem Commun (Camb). 2021 Feb 14;57(13):1603-1606. (PMID: 33463645)
Biomater Sci. 2020 Dec 15;8(24):6858-6866. (PMID: 32815940)
Acc Chem Res. 2020 Nov 17;53(11):2668-2679. (PMID: 33052654)
Biomacromolecules. 2014 Jan 13;15(1):122-31. (PMID: 24313776)
Chem Soc Rev. 2014;43(20):6881-93. (PMID: 25099656)
Chem Soc Rev. 2008 Apr;37(4):664-75. (PMID: 18362975)
Soft Matter. 2017 Jan 25;13(4):886. (PMID: 28090615)
Adv Healthc Mater. 2021 Jun;10(11):e2001903. (PMID: 33929772)
Adv Mater. 2020 Dec;32(51):e2002405. (PMID: 32989841)
Bioconjug Chem. 2017 May 17;28(5):1340-1350. (PMID: 28379682)
J Pept Sci. 2014 Jul;20(7):453-67. (PMID: 24729276)
Angew Chem Int Ed Engl. 2016 Sep 19;55(39):12094-8. (PMID: 27558471)
Angew Chem Int Ed Engl. 2007;46(43):8128-47. (PMID: 17935097)
Nat Biotechnol. 2003 Oct;21(10):1171-8. (PMID: 14520402)
Adv Mater. 2020 Oct;32(41):e1905669. (PMID: 32009269)
Biopolymers. 1990 May-Jun;29(6-7):1045-56. (PMID: 2369614)
Langmuir. 2019 Aug 20;35(33):10704-10724. (PMID: 31330107)
Nanoscale. 2013 Jul 21;5(14):6413-21. (PMID: 23739953)
Int J Nanomedicine. 2018 Sep 03;13:5003-5022. (PMID: 30214203)
Acc Chem Res. 2019 Sep 17;52(9):2634-2646. (PMID: 31478643)
Chem Commun (Camb). 2018 Sep 20;54(76):10730-10733. (PMID: 30191235)
Org Biomol Chem. 2015 Jan 28;13(4):1030-9. (PMID: 25410414)
Biochemistry. 2008 Apr 22;47(16):4597-605. (PMID: 18370402)
Nanoscale. 2020 Jun 11;12(22):11858-11862. (PMID: 32484195)
Langmuir. 2017 Apr 4;33(13):3234-3240. (PMID: 28282150)
J Am Chem Soc. 2014 Jun 18;136(24):8540-3. (PMID: 24911245)
Beilstein J Org Chem. 2020 Aug 17;16:2017-2025. (PMID: 32874348)
J Struct Biol. 1995 Jan-Feb;114(1):23-7. (PMID: 7772415)
ACS Nano. 2011 Sep 27;5(9):6791-818. (PMID: 21870803)
Chemistry. 2018 Sep 25;24(54):14316-14328. (PMID: 29667727)
Nano Today. 2016 Feb;11(1):41-60. (PMID: 27103939)
J Phys Chem B. 2019 Jul 11;123(27):5909-5915. (PMID: 31246033)
Chem Sci. 2020 Jun 2;11(26):6780-6788. (PMID: 32874522)
Biomater Sci. 2019 Apr 23;7(5):2023-2036. (PMID: 30839983)
Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202113403. (PMID: 34758508)
Biomacromolecules. 2008 Jun;9(6):1511-8. (PMID: 18498190)
J Am Chem Soc. 2007 Nov 28;129(47):14793-9. (PMID: 17985907)
J Am Chem Soc. 2018 Jan 17;140(2):534-537. (PMID: 29271649)
Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1428-32. (PMID: 8108426)
ACS Appl Mater Interfaces. 2020 Nov 25;12(47):52445-52456. (PMID: 33190483)
Angew Chem Int Ed Engl. 2021 Apr 26;60(18):9973-9977. (PMID: 33605524)
Sci Rep. 2014 Mar 12;4:4293. (PMID: 24618744)
Chempluschem. 2020 May;85(5):1022-1033. (PMID: 32421254)
Science. 1991 Nov 29;254(5036):1312-9. (PMID: 1962191)
Chemistry. 2011 Aug 8;17(33):9016-26. (PMID: 21726009)
Molecules. 2021 Feb 18;26(4):. (PMID: 33670668)
Angew Chem Int Ed Engl. 2020 Sep 28;59(40):17517-17524. (PMID: 32537822)
Acta Biochim Biophys Sin (Shanghai). 2007 Aug;39(8):549-59. (PMID: 17687489)
Nat Mater. 2016 Apr;15(4):469-76. (PMID: 26779883)
Cancers (Basel). 2021 Mar 09;13(5):. (PMID: 33803133)
Trends Biotechnol. 2012 Mar;30(3):155-65. (PMID: 22197260)
ACS Macro Lett. 2020 Jun 16;9(6):882-888. (PMID: 35648521)
Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5133-8. (PMID: 11929981)
Chem Soc Rev. 2016 Oct 21;45(20):5589-5604. (PMID: 27487936)
Annu Rev Biomed Eng. 2012;14:1-16. (PMID: 22524388)

ERC-StG-2016 SUPRACOP-715923 H2020 European Research Council

Keywords: amphiphilic systems; nanostructures; peptides; secondary structures; self-assembly

0 (Peptides)
0 (Anti-Bacterial Agents)

Date Created: 20211110 Date Completed: 20240724 Latest Revision: 20240901

20250114

PMC9300061

10.1002/anie.202113403

34758508