Tea Seed Kaempferol Triglycoside Attenuates LPS-Induced Systemic Inflammation and Ameliorates Cognitive Impairments in a Mouse Model.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

Cognitive Dysfunction*/drug therapy ; Lipopolysaccharides*/adverse effects; Animals ; Anti-Inflammatory Agents/therapeutic use ; Cytokines/metabolism ; Disease Models, Animal ; Inflammation/metabolism ; Kaempferols ; Mice ; Tea/chemistry

(1) Background: The current research intended to obtain functional compounds from agricultural by-products. A functional tea seed flavonoid, kaempferol-3-O-[2-O-β-d-xylopyranosyl-6-O-α-L-rhanmopyranosyl]-β-d-glucopyranoside (KXRG), was isolated from tea seed dregs. We further determined its chemical structure and evaluated the protective effects of KXRG against local and systemic inflammation in vivo; (2) Methods: First, cytotoxicity and proinflammatory cytokine release were examined in a cell-culture system. The biological activities of KXRG were investigated in a mouse model of ear edema, and from inflammatory damage to organs as demonstrated by histologic examination, in addition to brain function evaluation using the Y-maze test. Serum biochemical analysis and western blotting were utilized to explore the related cellular factors; (3) Results: KXRG inhibited IL-6 in RAW264.7 cells at a non-toxic concentration. Further experiments confirmed that KXRG exerted a stronger effect than indomethacin in terms of the prevention of 12-O-tetradecanoylphorbol acetate (TPA)-induced ear inflammation in a mouse model. KXRG feeding significantly prevented LPS-induced small intestine, liver, and kidney inflammatory damage, as demonstrated by histologic examination. KXRG also significantly improved LPS-induced cognitive impairments. Serum biochemical analysis showed that KXRG elevated antioxidant capacity and reduced levels of proinflammatory cytokines. Western blotting revealed that KXRG reduced the COX-2 expression induced by LPS in mouse tissues; (4) Conclusions: KXRG can be purified from agricultural waste, and hence it is inexpensive, with large amounts of raw materials available. Thus, KXRG has strong potential for further development as a wide-use anti-systemic inflammation drug to prevent human disease.

Front Aging Neurosci. 2018 Sep 18;10:279. (PMID: 30279656)
Int J Mol Sci. 2019 Nov 28;20(23):. (PMID: 31795299)
Nature. 1993 Jan 7;361(6407):31-9. (PMID: 8421494)
Nutrients. 2018 Dec 01;10(12):. (PMID: 30513729)
Acta Biochim Biophys Sin (Shanghai). 2014 Oct;46(10):920-2. (PMID: 25189429)
Hepatology. 1995 Mar;21(3):855-62. (PMID: 7875684)
Molecules. 2021 Apr 09;26(8):. (PMID: 33918918)
Front Physiol. 2021 May 28;12:660552. (PMID: 34122132)
Molecules. 2020 Feb 10;25(3):. (PMID: 32050623)
Int J Mol Sci. 2015 Nov 02;16(11):26087-124. (PMID: 26540040)
Nucleic Acids Res. 1985 Sep 11;13(17):6361-73. (PMID: 2995927)
Int Immunopharmacol. 2008 Sep;8(9):1264-71. (PMID: 18602073)
Antiinflamm Antiallergy Agents Med Chem. 2016;15(2):74-90. (PMID: 27634207)
Medchemcomm. 2019 Jul 26;10(10):1819-1827. (PMID: 31814955)
J Immunol. 1988 Dec 1;141(11):3875-81. (PMID: 3263439)
Asian Pac J Cancer Prev. 2014;15(14):5501-8. (PMID: 25081655)
Gastroenterology. 1988 Dec;95(6):1588-94. (PMID: 2846404)
J Alzheimers Dis. 2003 Apr;5(2):105-17. (PMID: 12719628)
Prostaglandins Other Lipid Mediat. 2002 Aug;68-69:95-114. (PMID: 12432912)
Lab Anim. 2015 Apr;49(1 Suppl):37-46. (PMID: 25835737)
Biomol Ther (Seoul). 2019 May 1;27(3):241-253. (PMID: 31006180)
Neurosci Res. 2008 May;61(1):113-9. (PMID: 18336945)
Mediators Inflamm. 2007;2007:45673. (PMID: 18274639)
Toxicol Appl Pharmacol. 2019 Feb 1;364:22-28. (PMID: 30528763)
Front Pharmacol. 2019 Oct 31;10:1295. (PMID: 31749703)
FASEB J. 1990 Aug;4(11):2860-7. (PMID: 2199284)
Molecules. 2011 Apr 18;16(4):3338-44. (PMID: 21512441)
Saudi Pharm J. 2019 May;27(4):507-516. (PMID: 31061619)
Sci Rep. 2019 Apr 8;9(1):5790. (PMID: 30962497)
J Biol Chem. 1988 May 5;263(13):6177-82. (PMID: 2452159)
Food Chem. 2014 Aug 1;156:369-73. (PMID: 24629982)
Circ Shock. 1994 Jan;42(1):53-8. (PMID: 8149510)
Mar Drugs. 2021 Sep 18;19(9):. (PMID: 34564186)
Front Physiol. 2016 Jan 26;6:363. (PMID: 26858648)
BMC Complement Altern Med. 2018 Aug 3;18(1):230. (PMID: 30075775)
Virology. 2010 Apr 25;400(1):104-14. (PMID: 20170934)
Annu Rev Immunol. 1985;3:263-87. (PMID: 2933048)
J Food Sci. 2019 Dec;84(12):3555-3564. (PMID: 31721202)
Exp Mol Med. 2009 Sep 30;41(9):629-37. (PMID: 19478557)
Shock. 2010 Dec;34(6):608-14. (PMID: 20442692)
Pharmacol Res. 2004 Jan;49(1):37-43. (PMID: 14597150)
Innate Immun. 2012 Feb;18(1):70-9. (PMID: 21239456)
Cold Spring Harb Perspect Biol. 2009 Dec;1(6):a001651. (PMID: 20457564)
J Nutr Sci Vitaminol (Tokyo). 2010;56(1):60-7. (PMID: 20354348)
Signal Transduct Target Ther. 2017;2:. (PMID: 29158945)
J Clin Invest. 2001 Jan;107(1):7-11. (PMID: 11134171)

Keywords: LPS; Y-maze; ear; flavonoid; human disease; small intestine

0 (Anti-Inflammatory Agents)
0 (Cytokines)
0 (Kaempferols)
0 (Lipopolysaccharides)
0 (Tea)
731P2LE49E (kaempferol)

Date Created: 20220412 Date Completed: 20220413 Latest Revision: 20220531

20250114

PMC9000603

10.3390/molecules27072055

35408453