Culicoides species community composition and feeding preferences in two aquatic ecosystems in northern Spain.

Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE

Original Publication: London : BioMed Central

Ceratopogonidae*/genetics ; Deer*; Animals ; Birds ; Ecosystem ; Feeding Behavior ; Female ; Spain

Background: Aquatic ecosystems provide breeding sites for blood-sucking insects such as Culicoides biting midges (Diptera: Ceratopogonidae), but factors affecting their distribution and host choice are poorly understood. A study was undertaken at two nature reserves in northern Spain to examine the abundance, species composition, population dynamics and feeding patterns of biting midges between 2018 and 2019.
Methods: Culicoides were captured by light suction traps baited with CO 2 and by sweep netting vegetation. Blood meals and species identification of blood-fed specimens were determined using cytochrome c oxidase I subunit (COI) DNA barcoding. Multivariate generalized linear models were used to evaluate the associations between the abundance of Culicoides, the species richness and other parameters.
Results: The 4973 identified specimens comprised 28 species of Culicoides. These included two species reported for the first time in northern Spain, thus raising to 54 the number of Culicoides species described in the region. Specimens of all 28 species and 99.6% of the total specimens collected were caught in suction traps, while sweep netting vegetation revealed just 11 species and 0.4% of the total specimens. Midge abundance peaked in June/early July, with five species comprising > 80% of the captures: Culicoides alazanicus (24.9%), Culicoides griseidorsum (20.3%), Culicoides poperinghensis (16.2%), Culicoides kibunensis (10.7%) and Culicoides clastrieri (9.6%). DNA barcode analysis of blood meals from eight Culicoides species revealed that they fed on 17 vertebrate species (3 mammals and 14 birds). Species in the subgenus Avaritia were primarily ornithophilic, except for C. griseidorsum and C. poperinghensis. Host DNA from blood meals was successfully amplified from 75% of blood-fed females. A pictorial blood meal digestion scale is provided to accurately assess the blood-fed status of female Culicoides.
Conclusions: The large number of different blood meal sources identified in the midges captured in this study signals the likely importance of wild birds and mammals (e.g. red deer and wild boar) as reservoir/amplifying hosts for pathogens. Available hosts are more exposed to being bitten by biting midge populations in aquatic ecosystems in late spring and early summer.
(© 2022. The Author(s).)

Annu Rev Entomol. 2015 Jan 7;60:373-92. (PMID: 25386725)
Med Vet Entomol. 2019 Dec;33(4):467-475. (PMID: 31099060)
Parasit Vectors. 2018 Oct 1;11(1):524. (PMID: 30269688)
Bull Entomol Res. 2012 Dec;102(6):692-7. (PMID: 22647415)
Med Vet Entomol. 2015 Sep;29(3):290-6. (PMID: 25689114)
J Insect Sci. 2020 Nov 1;20(6):. (PMID: 33135756)
J Med Entomol. 2022 Mar 16;59(2):772-776. (PMID: 34971396)
Parasit Vectors. 2020 Feb 17;13(1):79. (PMID: 32066493)
Trends Parasitol. 2015 Jan;31(1):16-22. (PMID: 25435248)
Emerg Infect Dis. 2012 Jul;18(7):1204-6. (PMID: 22709978)
Sci Rep. 2021 Jan 13;11(1):521. (PMID: 33441647)
Parasit Vectors. 2015 Feb 08;8:86. (PMID: 25886488)
Front Ecol Evol. 2020 Jul;8:. (PMID: 32775339)
Vet Parasitol. 2013 Jan 16;191(1-2):81-93. (PMID: 22999100)
PLoS One. 2012;7(2):e31098. (PMID: 22363557)
Parasit Vectors. 2018 Mar 27;11(1):217. (PMID: 29587832)
Parasitol Res. 2011 Oct;109(4):1133-41. (PMID: 21461726)
Annu Rev Entomol. 2000;45:307-40. (PMID: 10761580)
Mol Ecol Resour. 2014 May;14(3):508-18. (PMID: 24299419)
Med Vet Entomol. 2013 Mar;27(1):104-12. (PMID: 22882691)
Parasitol Res. 2009 Aug;105(2):373-80. (PMID: 19308450)
Antiviral Res. 2013 Oct;100(1):102-13. (PMID: 23933421)
Parasit Vectors. 2012 Jul 24;5:147. (PMID: 22827913)
Parasitol Res. 2011 Mar;108(3):657-63. (PMID: 20967462)
Parasit Vectors. 2020 Apr 15;13(1):194. (PMID: 32295627)
Vet Ital. 2017 Jun 30;53(2):157-166. (PMID: 28675253)
PLoS One. 2013 Jun 14;8(6):e66893. (PMID: 23799137)
Med Vet Entomol. 2018 Mar;32(1):35-40. (PMID: 28857265)
Acta Trop. 2016 May;157:59-67. (PMID: 26826391)
Parasitol Res. 2015 Apr;114(4):1607-10. (PMID: 25656463)
Zookeys. 2014 Dec 12;(463):95-131. (PMID: 25589864)
Parasit Vectors. 2020 Aug 3;13(1):393. (PMID: 32746908)
Infect Genet Evol. 2011 Jul;11(5):1103-10. (PMID: 21511056)
Parasitology. 2013 Dec;140(14):1799-810. (PMID: 23981661)
Parasit Vectors. 2012 Jul 23;5:143. (PMID: 22824422)
Vet Parasitol. 2009 Nov 12;165(3-4):298-310. (PMID: 19682796)
Mol Biol Evol. 2013 Dec;30(12):2725-9. (PMID: 24132122)
Parasit Vectors. 2020 Mar 16;13(1):139. (PMID: 32178710)
Med Vet Entomol. 2010 Jun;24(2):199-209. (PMID: 20604864)
PLoS Negl Trop Dis. 2020 Dec 31;14(12):e0008867. (PMID: 33382725)
Infect Genet Evol. 2010 Jul;10(5):629-37. (PMID: 20381646)
Parasit Vectors. 2017 Mar 27;10(1):163. (PMID: 28347323)
Front Vet Sci. 2015 May 27;2:15. (PMID: 26664944)
Parasite. 2007 Jun;14(2):173-7. (PMID: 17645192)
Parasit Vectors. 2018 Feb 27;11(1):112. (PMID: 29482593)
Parasit Vectors. 2020 May 20;13(1):265. (PMID: 32434592)
Parasit Vectors. 2019 Jan 25;12(1):61. (PMID: 30683145)
Parasit Vectors. 2012 Jul 09;5:137. (PMID: 22776566)

FPI-2019 Department of Economic Development and Infrastructures of the Basque Government; FJC2019-041737-I 'Juan de la Cierva-Formación' post-doctoral grant - MCIN/AEI/ 10.13039/501100011033; SV3045 The Scottish Government and Welsh Government

Keywords: Barcoding; Biting midges; Dynamic populations; Freshwater habitats; Host blood meals; Species richness

Date Created: 20220611 Date Completed: 20220614 Latest Revision: 20220716

20240829

PMC9188056

10.1186/s13071-022-05297-5

35690834