Isolation, purification and biochemical characterization of Conopomorpha cramerella farnesol dehydrogenase.

Publisher: Blackwell Scientific For The Royal Entomological Society Country of Publication: England NLM ID: 9303579 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2583 (Electronic) Linking ISSN: 09621075 NLM ISO Abbreviation: Insect Mol Biol Subsets: MEDLINE

Publication: Oxford : Blackwell Scientific For The Royal Entomological Society
Original Publication: Oxford : Published for the Royal Entomological Society by Blackwell Scientific Publications, c1992-

Farnesol*/metabolism ; Insecticides*; Humans ; Animals ; Phylogeny ; Acetone ; NADP ; Insecta/metabolism

In Southeast Asia, Conopomorpha cramerella (Snellen) which is commonly known as the cocoa pod borer (CPB) moth has been identified as the most detrimental pest of Theobroma cacao L. Apart from the various side effects on human health and non-target organisms, heavily relying on synthetic pyrethroid insecticides to control CPB infestations also increases the environmental contamination risks. Thus, developing biorational insecticides that minimally affect the non-target organism and environment by targeting the insect growth regulation process is needed to manage the pest population. In insects, juvenile hormones (JH) regulate critical biological events, especially metamorphosis, development and reproduction. Since the physiological roles of JH III vary among different organisms, the biochemical properties, especially substrate specificity and analogue inhibition, may also be different. Therefore, studies on the JH III biosynthetic pathway enzymes in both plants and insects are beneficial to discover more effective analogues. Bioinformatic analysis and biochemical characterization of a NADP ⁺ -dependent farnesol dehydrogenase, an intermediate enzyme of the JH III pathway, from C. cramerella (CcFolDH), were described in this study. In addition, the farnesol analogues that may act as a potent analogue inhibitor for CcFolDH ware determined using in vitro enzymatic study. The phylogenetic analysis indicated that CcFolDH shared a close phylogenetic relationship to the honeybee's short-chain dehydrogenase/reductase. The 27 kDa CcFolDH has an NADP(H) binding domain with a typical Rossmann fold and is likely a homotetrameric protein in the solution. The enzyme had a greater preference for substrate trans, trans-farnesol and coenzyme NADP ⁺ . In terms of analogue inhibitor inhibition, hexahydroxyfarnesyl acetone showed the highest inhibition (the lowest K i ) compared to other farnesol analogues. Thus, hexahydroxyfarnesyl acetone would serve as the most potent active ingredient for future biorational pesticide management for C. cramerella infestation. Based on the bioinformatic analyses and biochemical characterizations conducted in this research, we proposed that rCcFolDH differs slightly from other reported farnesol dehydrogenases in terms of molecular weight, substrate preference, coenzymes utilization and analogue inhibitors selection.
(© 2022 Royal Entomological Society.)

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Keywords: Conopomorpha cramerella; biochemical characterization; farnesol dehydrogenase; structural modelling

EC 1.1.1.216 (farnesol dehydrogenase)
4602-84-0 (Farnesol)
0 (Insecticides)
1364PS73AF (Acetone)
53-59-8 (NADP)
B74U6BJ6J5 (juvenile hormone III)

Date Created: 20221201 Date Completed: 20230314 Latest Revision: 20230316

20240513

10.1111/imb.12820

36454188