Alphavirus Particles Can Assemble with an Alternate Triangulation Number.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI

Alphavirus*/genetics ; Encephalitis Virus, Eastern Equine*/genetics; Capsid Proteins/genetics ; Virion/genetics

Alphaviruses are spherical, enveloped RNA viruses primarily transmitted by mosquitoes, and cause significant arthritogenic and neurotropic disease in humans and livestock. Previous reports have shown that-in contrast to prototypical icosahedral viruses-alphaviruses incorporate frequent defects, and these may serve important functions in the viral life cycle. We confirm the genus-wide pleomorphism in live viral particles and extend our understanding of alphavirus assembly through the discovery of an alternate architecture of Eastern equine encephalitis virus (EEEV) particles. The alternate T = 3 icosahedral architecture differs in triangulation number from the classic T = 4 icosahedral organization that typifies alphaviruses, but the alternate architecture maintains the quasi-equivalence relationship of asymmetric units. The fusion spike glycoproteins are more loosely apposed in the T = 3 form with corresponding changes in the underlying capsid protein lattice. This alternate architecture could potentially be exploited in engineering alphavirus-based particles for delivery of alphaviral or other RNA.

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R01 AI153433 United States AI NIAID NIH HHS; K22AI125474 National Institute of Allergy and Infectious Diseases; R01AI153433 National Institute of Allergy and Infectious Diseases; P41GM103832 United States GM NIGMS NIH HHS

Keywords: Togaviridae; alphavirus; cryo-electron microscopy; quasi-equivalence; triangulation number; virus assembly

0 (Capsid Proteins)

Date Created: 20221223 Date Completed: 20230105 Latest Revision: 20230417

20250114

PMC9780915

10.3390/v14122650

36560655