Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Genomic Characterization of the Historical Smallpox Vaccine Strain Wyeth Isolated from a 1971 Seed Vial.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI
    • Subject Terms:
      Smallpox*/prevention & control ; Vaccinia virus*/genetics; Humans ; Genomics ; Smallpox Vaccine
    • Abstract:
      The Wyeth strain of vaccinia virus (VACV) produced by Wyeth Pharmaceuticals was supposedly used to manufacture the old freeze-dried American smallpox vaccine, Dryvax, until its discontinuation in 2008. Although the genomic sequences of numerous Dryvax clones have been reported, data on VACV-Wyeth genomes are still lacking. Genomic analysis of old VACV strains is relevant to understand the evolutionary relationships of smallpox vaccines, particularly with the recent resumption of smallpox vaccination in certain population groups as an attempt to control the worldwide monkeypox outbreak. Here we analyzed the complete genome sequences of three VACV-Wyeth clonal isolates obtained from a single seed vial donated to the Brazilian eradication program in the 1970s. Wyeth clones show >99.3% similarity to each other and >95.3% similarity with Dryvax clones, mapping together in clade I of the vaccinia group. Although the patterns of SNPs and INDELs comparing Dryvax and Wyeth clones are overall uniform, important differences were detected particularly at the ends of the genome. In addition, we detected recombinant events of clone Wyeth A111 and the Dryvax clone Acam2000, suggesting that other regions of the genomes may have similar patchy patterns of recombination. A small-scale serological survey using VACV-Wyeth as antigen in ELISA assays revealed that 63 of the 65 individuals born before the end of smallpox vaccination in Brazil still have anti-VACV IgG antibodies, demonstrating the usefulness of the VACV-Wyeth strain in future extended serological studies of the Brazilian population.
    • References:
      Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16262-7. (PMID: 20805472)
      Vaccine. 2007 Dec 17;25(52):8807-32. (PMID: 18037545)
      Vaccine. 2020 Jun 19;38(30):4773-4779. (PMID: 32473878)
      J Virol. 1999 Jan;73(1):152-60. (PMID: 9847317)
      Lancet Reg Health Am. 2022 Sep;13:100315. (PMID: 36118618)
      PLoS Pathog. 2018 Jul 26;14(7):e1007082. (PMID: 30048524)
      J Virol. 2014 May;88(10):5277-86. (PMID: 24574414)
      Mem Inst Oswaldo Cruz. 2021 Feb 03;115:e200521. (PMID: 33566940)
      NPJ Vaccines. 2022 Aug 11;7(1):92. (PMID: 35953491)
      J Virol. 2015 Feb;89(3):1809-24. (PMID: 25410873)
      J Virol. 2017 Nov 14;91(23):. (PMID: 28904196)
      Mol Biol Evol. 2018 Jun 1;35(6):1547-1549. (PMID: 29722887)
      Can J Public Health. 2010 Mar-Apr;101(2):113-4, 118. (PMID: 20524373)
      Nature. 2004 Mar 11;428(6979):182-5. (PMID: 15014500)
      Bioinformatics. 2010 Mar 1;26(5):589-95. (PMID: 20080505)
      J Virol. 2015 Dec;89(23):11909-25. (PMID: 26378174)
      Acta Trop. 2002 Aug;83(2):159-68. (PMID: 12088857)
      BMC Genomics. 2006 Jun 13;7:150. (PMID: 16772042)
      Biotechnol Genet Eng Rev. 2018 Apr;34(1):107-121. (PMID: 29779454)
      Genome Biol. 2020 Jul 20;21(1):175. (PMID: 32684155)
      Vaccine. 2009 Jan 29;27(5):708-17. (PMID: 19059294)
      Genome Biol. 2020 Dec 4;21(1):286. (PMID: 33272280)
      Pathogens. 2022 Aug 09;11(8):. (PMID: 36015016)
      PLoS Med. 2006 Aug;3(8):e272. (PMID: 16933957)
      Genome Announc. 2016 May 12;4(3):. (PMID: 27174282)
      Antiviral Res. 2009 Oct;84(1):1-13. (PMID: 19563829)
      J Virol. 2011 Dec;85(24):13049-60. (PMID: 21976639)
      J Gen Virol. 2007 Jul;88(Pt 7):1906-1916. (PMID: 17554021)
      Am J Vet Res. 1964 Jan;25:192-5. (PMID: 14103224)
      Int J Infect Dis. 2004 Oct;8 Suppl 2:S31-44. (PMID: 15491873)
      Future Microbiol. 2010 Sep;5(9):1367-82. (PMID: 20860482)
      Hist Cienc Saude Manguinhos. 2010;17(3):777-90. (PMID: 21461539)
      Lancet Infect Dis. 2022 Nov 7;:. (PMID: 36356606)
      Lancet Infect Dis. 2022 Dec;22(12):e349-e358. (PMID: 36116460)
      Nat Med. 2022 Oct 18;:. (PMID: 36257333)
      N Engl J Med. 2017 Oct 12;377(15):1491-1492. (PMID: 29020595)
      Viruses. 2018 Nov 15;10(11):. (PMID: 30445717)
      PLoS One. 2013 Apr 12;8(4):e60557. (PMID: 23593246)
      Science. 2022 Aug 12;377(6607):696-697. (PMID: 35951681)
      Lancet Infect Dis. 2018 Feb;18(2):e55-e63. (PMID: 28827144)
    • Contributed Indexing:
      Keywords: monkeypox virus; poxvirus; smallpox vaccine; vaccinia virus
    • Accession Number:
      0 (Smallpox Vaccine)
    • Publication Date:
      Date Created: 20230121 Date Completed: 20230125 Latest Revision: 20231116
    • Publication Date:
      20250114
    • Accession Number:
      PMC9864299
    • Accession Number:
      10.3390/v15010083
    • Accession Number:
      36680123