Bioaerosols in swine confinement buildings: A metaproteomic view.

Publisher: Society for Applied Microbiology and Blackwell Pub Country of Publication: United States NLM ID: 101499207 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1758-2229 (Electronic) Linking ISSN: 17582229 NLM ISO Abbreviation: Environ Microbiol Rep Subsets: MEDLINE

Original Publication: [Hoboken, N.J.] : Society for Applied Microbiology and Blackwell Pub., c2009-

Air Microbiology* ; Bacteria*/genetics; Animals ; Swine ; RNA, Ribosomal, 16S/genetics ; Aerosols ; Peptides

Swine confinement buildings represent workplaces with high biological air pollution. It is suspected that individual components of inhalable air are causatives of chronic respiratory disease that are regularly detected among workers. In order to understand the relationship between exposure and stress, it is necessary to study the components of bioaerosols in more detail. For this purpose, bioaerosols from pig barns were collected on quartz filters and analysed via a combinatorial approach of 16S rRNA amplicon sequencing and metaproteomics. The study reveals the presence of peptides from pigs, their feed and microorganisms. The proportion of fungal peptides detected is considered to be underrepresented compared to bacterial peptides. In addition, the metaproteomic workflow enabled functional predictions about the discovered peptides. Housekeeping proteins were found in particular, but also evidence for the presence of bacterial virulence factors (e.g., serralysin-like metalloprotease) as well as plant (e.g., chitinase) and fungal allergens (e.g., alt a10). Metaproteomic analyses can thus be used to identify factors that may be relevant to the health of pig farmers. Accordingly, such studies could be used in the future to assess the adverse health potential of an occupationally relevant bioaerosol and help consider defined protective strategies for workers.
(© 2023 Bundesanstalt für Arbeitsschutz und Arbeitsmedizin and The Authors. Environmental Microbiology Reports published by Applied Microbiology International and John Wiley & Sons Ltd.)

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0 (RNA, Ribosomal, 16S)
0 (Aerosols)
0 (Peptides)

Date Created: 20231102 Date Completed: 20231127 Latest Revision: 20231127

20231215

PMC10667663

10.1111/1758-2229.13208

37919246