Label-Free SERS and MD Analysis of Biomarkers for Rapid Point-of-Care Sensors Detecting Head and Neck Cancer and Infections.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101204366 Publication Model: Electronic Cited Medium: Internet ISSN: 1424-8220 (Electronic) Linking ISSN: 14248220 NLM ISO Abbreviation: Sensors (Basel) Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c2000-

Mouth Neoplasms*/diagnosis ; Carcinoma, Squamous Cell*/diagnosis ; Head and Neck Neoplasms*/diagnosis; Humans ; Point-of-Care Systems ; Biomarkers/analysis ; Saliva/chemistry ; Spectrum Analysis, Raman ; Biomarkers, Tumor/analysis

For the progress of point-of-care medicine, where individual health status can be easily and quickly monitored using a handheld sensor, saliva serves as one of the best-suited body fluids thanks to its availability and abundance of physiological indicators. Salivary biomarkers, combined with rapid and highly sensitive detection tools, may pave the way to new real-time health monitoring and personalized preventative therapy branches using saliva as a target matrix. Saliva is increasing in importance in liquid biopsy, a non-invasive approach that helps physicians diagnose and characterize specific diseases in patients. Here, we propose a proof-of-concept study combining the unique specificity in biomolecular recognition provided by surface-enhanced Raman spectroscopy (SERS) in combination with molecular dynamics (MD) simulations, which give leave to explore the biomolecular absorption mechanism on nanoparticle surfaces, in order to verify the traceability of two validated salivary indicators, i.e., interleukin-8 (IL-8) and lysozyme (LYZ), implicated in oropharyngeal squamous cell carcinoma (OSCC) and oral infection. This strategy simultaneously assures the detection and interpretation of protein biomarkers in saliva, ultimately opening a new route for the evolution of fast and accurate point-of-care SERS-based sensors of interest in precision medicine diagnostics.

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860185 (PHAST = Photonics for Healthcare: multiscAle cancer diagnosiS and Therapy) European Union's Horizon 2020 research and innovation programme

Keywords: SERS; biomarkers; biosensors; cancer; infections; molecular dynamics; point-of-care; saliva

0 (Biomarkers)
0 (Biomarkers, Tumor)

Date Created: 20231114 Date Completed: 20231115 Latest Revision: 20231122

20240513

PMC10648186

10.3390/s23218915

37960614