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Investigation of the Efficacy of a Listeria monocytogenes Biosensor Using Chicken Broth Samples.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101204366 Publication Model: Electronic Cited Medium: Internet ISSN: 1424-8220 (Electronic) Linking ISSN: 14248220 NLM ISO Abbreviation: Sensors (Basel) Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, c2000-
    • Subject Terms:
      Listeria monocytogenes*/isolation & purification ; Biosensing Techniques*/methods ; Chickens* ; Food Microbiology*/methods ; Dielectric Spectroscopy*; Animals ; Electrodes
    • Abstract:
      Foodborne pathogens are microbes present in food that cause serious illness when the contaminated food is consumed. Among these pathogens, Listeria monocytogenes is one of the most serious bacterial pathogens, and causes severe illness. The techniques currently used for L. monocytogenes detection are based on common molecular biology tools that are not easy to implement for field use in food production and distribution facilities. This work focuses on the efficacy of an electrochemical biosensor in detecting L. monocytogenes in chicken broth. The sensor is based on a nanostructured electrode modified with a bacteriophage as a bioreceptor which selectively detects L. monocytogenes using electrochemical impedance spectroscopy. The biosensing platform was able to reach a limit of detection of 55 CFU/mL in 1× PBS buffer and 10 CFU/mL in 1% diluted chicken broth. The biosensor demonstrated 83-98% recovery rates in buffer and 87-96% in chicken broth.
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    • Grant Information:
      CFS2021 UGA Center for Food Safety
    • Contributed Indexing:
      Keywords: Listeria monocytogenes; bacteriophage; food safety; impedance; pathogen detection
    • Publication Date:
      Date Created: 20240427 Date Completed: 20240427 Latest Revision: 20240429
    • Publication Date:
      20240429
    • Accession Number:
      PMC11054734
    • Accession Number:
      10.3390/s24082617
    • Accession Number:
      38676242