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Enhanced effects of slowly co-released TGF-β3 and BMP-2 from biomimetic calcium phosphate-coated silk fibroin scaffolds in the repair of osteochondral defects.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101152208 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-3155 (Electronic) Linking ISSN: 14773155 NLM ISO Abbreviation: J Nanobiotechnology Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central, 2003-
    • Subject Terms:
      Fibroins*/chemistry ; Fibroins*/pharmacology ; Bone Morphogenetic Protein 2*/pharmacology ; Transforming Growth Factor beta3*/pharmacology ; Calcium Phosphates*/chemistry ; Calcium Phosphates*/pharmacology ; Tissue Scaffolds*/chemistry ; Bone Regeneration*/drug effects ; Tissue Engineering*/methods ; Osteogenesis*/drug effects; Animals ; Rabbits ; Chondrogenesis/drug effects ; Biomimetic Materials/chemistry ; Biomimetic Materials/pharmacology ; Bombyx ; Male
    • Abstract:
      Bioactive agents have demonstrated regenerative potential for cell-free bone tissue engineering. Nevertheless, certain challenges persist, including ineffective delivery methods and confined therapeutic potency. Here, we demonstrated that the biomimetic calcium phosphate coating system (BioCaP) could effectively uptake and slowly release the incorporated bioactive agents compared to the surface absorption system via osteoclast-mediated degradation of BioCaP coatings. The release kinetics were determined as a function of time. The release rate was stable without remarkable burst release during the first 1 day, followed by a sustained release from day 7 to day 19. Then, we developed the bi-functional BioCaP-coated silk fibroin scaffolds enabling the effective co-delivery of TGF-β3 and BMP-2 (SFI-T/SFI-B) and the corresponding slow release of TGF-β3 and BMP-2 exhibited superior potential in promoting chondrogenesis and osteogenesis without impairing cell vitality in vitro. The SFI-T/SFI-B scaffolds could improve cartilage and bone regeneration in 5 × 4 mm rabbit osteochondral (OC) defect. These findings indicate that the biomimetic calcium-phosphate coated silk fibroin scaffolds with slowly co-released TGF-β3 and BMP-2 effectively promote the repair of OC defects, hence facilitating the future clinical translation of controlled drug delivery in tissue engineering.
      (© 2024. The Author(s).)
    • Comments:
      Erratum in: J Nanobiotechnology. 2025 Jan 27;23(1):46. doi: 10.1186/s12951-025-03131-5.. (PMID: 39871309)
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    • Grant Information:
      81670960 National Natural Science Foundation of China; QNRC2016115 Jiangsu Provincial Medical Youth Talent; SBK2021021787 Natural Science Foundation of Jiangsu Province; ZKX20048 Top Young and Middle-aged Innovative Talents Program of Nanjing
    • Contributed Indexing:
      Keywords: Biomimetic calcium phosphate coating; Drug delivery; Osteochondral defect; Silk fibroin; Slow release; Tissue engineering
    • Accession Number:
      9007-76-5 (Fibroins)
      0 (Bone Morphogenetic Protein 2)
      0 (Transforming Growth Factor beta3)
      0 (Calcium Phosphates)
      97Z1WI3NDX (calcium phosphate)
    • Publication Date:
      Date Created: 20240731 Date Completed: 20240731 Latest Revision: 20250204
    • Publication Date:
      20250204
    • Accession Number:
      PMC11290091
    • Accession Number:
      10.1186/s12951-024-02712-0
    • Accession Number:
      39080653