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Identification and functional analysis of C-type lectin from mosquito Aedes albopictus in response to dengue virus infection.
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- Additional Information
- Source:
Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE
- Publication Information:
Original Publication: London : BioMed Central
- Subject Terms:
- Abstract:
Background: C-type lectins (CTLs) are a large family of proteins with sugar-binding activity. CTLs contain an evolutionarily conserved C-type lectin domain (CTLD) that binds microbial carbohydrates in a calcium-dependent manner, thereby playing a key role in both microbial pathogenesis and innate immune responses. Aedes albopictus is an important vector for transmitting dengue virus (DENV) worldwide. Currently, the molecular characteristics and functions of CTLs in Ae. albopictus are largely unknown.
Methods: Transcripts encoding CTL proteins in the Ae. albopictus genome assembly were analyzed via sequence blast. Phylogenetic analysis and molecular characterization were performed to identify the functional domains of the CTLs. Quantitative analysis was performed to determine the gene expression features of CTLs during mosquito development and in different tissues of female adults after blood feeding. In addition, the functional role of CTLs in response to DENV infection was investigated in Ae. albopictus mosquito cells.
Results: We identified 39 transcripts encoding CTL proteins in the Ae. albopictus transcriptome. Aedes albopictus CTLs are classified into three groups based on the number of CTLDs and the domain architecture. These included 29 CTL-Ss (single-CTLDs), 1 immulectins (dual-CTLD) and 9 CTL-Xs (CTLDs with other domains). Phylogenetic analysis and structural modeling indicated that CTLs in Ae. albopictus are highly conserved with the homologous CTLs in Aedes aegypti. The expression profile assay revealed differential expression patterns of CTLs in both developmental stages and in adult female tissues. Knockdown and overexpression of three CTLs (CTL-S12, S17 and S19) confirmed that they can promote dengue virus infection in Ae. albopictus cells.
Conclusions: The CTL genes in Ae. albopictus mosquito and other mosquito species are evolutionarily conserved and exhibit different developmental and tissue expression features. The functional assay indicated that three CTLs in Ae. albopictus mosquitoes are involved in promoting dengue virus infection. Our study revealed that CTLs play important roles in both the physiological processes and viral infection in mosquito vectors.
(© 2024. The Author(s).)
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- Grant Information:
No. 82172305 the National Natural Science Foundation of China; No. 82272371 the National Natural Science Foundation of China; No. LY21H190004 the Natural Science Foundation of Zhejiang Province
- Contributed Indexing:
Keywords: Aedes albopictus; C-type lectin; Dengue virus; Expression patterns; Functional study
- Accession Number:
0 (Lectins, C-Type)
0 (Insect Proteins)
- Subject Terms:
Aedes albopictus
- Publication Date:
Date Created: 20240905 Date Completed: 20240905 Latest Revision: 20240907
- Publication Date:
20250114
- Accession Number:
PMC11373435
- Accession Number:
10.1186/s13071-024-06453-9
- Accession Number:
39232769
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