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Photoactive nanocatalysts as DTT-assisted BSA-AuNCs with enhanced oxidase-mimicking ability for sensitive fluorometric detection of antioxidants.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101152208 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-3155 (Electronic) Linking ISSN: 14773155 NLM ISO Abbreviation: J Nanobiotechnology Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central, 2003-
    • Subject Terms:
      Serum Albumin, Bovine*/chemistry ; Antioxidants*/chemistry ; Antioxidants*/pharmacology ; Gold*/chemistry ; Metal Nanoparticles*/chemistry; Humans ; Catalysis ; Dithiothreitol/chemistry ; Saliva/chemistry ; Fluorometry/methods ; Oxidoreductases/metabolism ; Oxidoreductases/chemistry ; Limit of Detection ; Glutathione/chemistry ; Glutathione/metabolism ; Ascorbic Acid/chemistry ; Animals ; Oxidative Stress/drug effects ; Oxidation-Reduction
    • Abstract:
      Redox imbalance and oxidative stress are increasingly recognized as significant factors in health disorders such as neurodegenerative disorders, premature aging and cancer. However, detecting antioxidant levels that is crucial for managing oxidative stress, can be challenging due to existing assays' limitations, such as insensitivity to thiol-containing antioxidants. This study presents a simple fluorescence-based assay for antioxidant detection employing the enhanced photocatalytic oxidase-like activity of dithiothreitol (DTT)-assisted bovine serum albumin (BSA)-stabilized gold nanoclusters (DTT@BSA-AuNCs). The reported nanozyme exhibits remarkable stability, versatility, and catalytic activity. Under LED irradiation, DTT@BSA-AuNCs generate singlet oxygen, which converts non-fluorescent thiamine to fluorescent thiochrome, utilizing dissolved oxygen for catalysis. Antioxidants inhibit thiochrome formation, leading to fluorescence quenching. This method enables sensitive detection of antioxidants such as ascorbic acid and glutathione with limits of detection of 0.08 µM and 0.32 µM, respectively, under neutral pH, outperforming previous studies. The assay successfully detects antioxidants in human saliva and cancer cell models. The DTT@BSA-AuNCs-based assay offers a cost-effective, sensitive, and straightforward approach for detecting antioxidants in biological samples, facilitating improved monitoring of oxidative stress in various diseases.
      (© 2024. The Author(s).)
    • References:
      Biosens Bioelectron. 2015 Feb 15;64:165-70. (PMID: 25218100)
      J Mater Chem B. 2024 May 8;12(18):4502-4508. (PMID: 38646996)
      Bioconjug Chem. 2017 Dec 20;28(12):2903-2909. (PMID: 29172463)
      Biomolecules. 2020 Oct 09;10(10):. (PMID: 33050144)
      Sensors (Basel). 2020 Nov 07;20(21):. (PMID: 33171820)
      J Agric Food Chem. 2014 Feb 26;62(8):1839-45. (PMID: 24512512)
      Nanoscale Res Lett. 2013 Apr 15;8(1):170. (PMID: 23587362)
      Mikrochim Acta. 2018 Aug 3;185(8):400. (PMID: 30076470)
      Molecules. 2024 Apr 01;29(7):. (PMID: 38611853)
      Cancer. 1993 Aug 1;72(3):783-7. (PMID: 8392905)
      Colloids Surf B Biointerfaces. 2017 Jul 1;155:25-34. (PMID: 28391081)
      Clujul Med. 2014;87(4):215-9. (PMID: 26528027)
      Anal Chem. 2020 Jul 7;92(13):8660-8664. (PMID: 32483973)
      Food Chem. 2024 Jan 15;431:136908. (PMID: 37573743)
      RSC Adv. 2020 Sep 9;10(55):33299-33306. (PMID: 35515024)
      Analyst. 2021 Aug 9;146(16):5061-5066. (PMID: 34296710)
      Mikrochim Acta. 2019 May 16;186(6):354. (PMID: 31098776)
      ACS Omega. 2019 Jun 10;4(6):10070-10077. (PMID: 31460099)
      ACS Nano. 2023 May 9;17(9):8167-8182. (PMID: 37083341)
      Postepy Hig Med Dosw (Online). 2006;60:170-80. (PMID: 16618987)
      Analyst. 2020 Jul 7;145(13):4388-4397. (PMID: 32420572)
      Nat Nanotechnol. 2007 Sep;2(9):577-83. (PMID: 18654371)
      Clin Dev Immunol. 2012;2012:708036. (PMID: 22693526)
      Arch Toxicol. 2023 Oct;97(10):2499-2574. (PMID: 37597078)
      Mikrochim Acta. 2024 Mar 21;191(4):214. (PMID: 38512502)
      Nat Cell Biol. 2016 May;18(5):572-8. (PMID: 27088857)
      Chem Commun (Camb). 2012 Mar 28;48(26):3221-3. (PMID: 22354149)
      ACS Appl Mater Interfaces. 2016 Aug 31;8(34):22301-8. (PMID: 27503256)
      Clin Chim Acta. 2002 Nov;325(1-2):165-70. (PMID: 12367782)
      Anal Bioanal Chem. 2018 Nov;410(28):7385-7394. (PMID: 30215122)
      Chem Soc Rev. 2019 Feb 18;48(4):1004-1076. (PMID: 30534770)
      Molecules. 2007 Jul 19;12(7):1496-547. (PMID: 17909504)
      Prog Biophys Mol Biol. 2018 Dec;140:5-12. (PMID: 29530789)
      Arch Biochem Biophys. 2021 Nov 30;713:109061. (PMID: 34662556)
      Mol Syst Des Eng. 2017 Dec 1;2(5):624-628. (PMID: 29430303)
      Acc Chem Res. 2021 Mar 2;54(5):1274-1287. (PMID: 33570397)
      Free Radic Res. 2015 May;49(5):633-49. (PMID: 25511471)
      Biosens Bioelectron. 2016 May 15;79:467-75. (PMID: 26745793)
      Oxid Med Cell Longev. 2013;2013:972913. (PMID: 23766865)
      J Agric Food Chem. 2005 May 18;53(10):4290-302. (PMID: 15884874)
      Materials (Basel). 2022 Nov 27;15(23):. (PMID: 36499945)
      ACS Appl Bio Mater. 2024 May 20;7(5):2695-2703. (PMID: 38701372)
      ChemistryOpen. 2017 Mar 08;6(2):178-191. (PMID: 28413748)
      Nat Commun. 2022 Nov 9;13(1):6758. (PMID: 36351899)
      J Photochem Photobiol B. 2020 Jan;203:111766. (PMID: 31927488)
      Anal Chem. 2019 Jul 2;91(13):8170-8175. (PMID: 31148448)
      Molecules. 2023 Jan 20;28(3):. (PMID: 36770724)
      Theranostics. 2017 Jun 1;7(8):2277-2286. (PMID: 28740550)
      J Am Chem Soc. 2009 Jan 28;131(3):888-9. (PMID: 19123810)
      Nat Commun. 2023 Aug 22;14(1):5083. (PMID: 37607944)
      Mater Today Bio. 2023 May 04;20:100656. (PMID: 37214551)
      J Phys Chem B. 2022 Sep 15;126(36):6792-6801. (PMID: 36044985)
      ACS Appl Mater Interfaces. 2020 Mar 18;12(11):12525-12532. (PMID: 32106677)
    • Grant Information:
      111-2112-M-008 -021 -MY3 National Science and Technology Council; 111-2112-M-008 -021 -MY3 National Science and Technology Council; 111-2112-M-008 -021 -MY3 National Science and Technology Council; 111-2112-M-008 -021 -MY3 National Science and Technology Council; 111-2112-M-008 -021 -MY3 National Science and Technology Council; 111-2112-M-008 -021 -MY3 National Science and Technology Council; 111-2112-M-008 -021 -MY3 National Science and Technology Council
    • Contributed Indexing:
      Keywords: Antioxidants; DTT@BSA-AuNCs; Fluorescence; Oxidase-like nanozymes; Photocatalysis
    • Accession Number:
      27432CM55Q (Serum Albumin, Bovine)
      0 (Antioxidants)
      7440-57-5 (Gold)
      T8ID5YZU6Y (Dithiothreitol)
      EC 1.- (Oxidoreductases)
      GAN16C9B8O (Glutathione)
      PQ6CK8PD0R (Ascorbic Acid)
    • Publication Date:
      Date Created: 20240928 Date Completed: 20240929 Latest Revision: 20241001
    • Publication Date:
      20250114
    • Accession Number:
      PMC11438146
    • Accession Number:
      10.1186/s12951-024-02850-5
    • Accession Number:
      39342215