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Molecular condensation of the CO/NF-YB/NF-YC/FT complex gates floral transition in Arabidopsis.

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  • Additional Information
    • Source:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 8208664 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-2075 (Electronic) Linking ISSN: 02614189 NLM ISO Abbreviation: EMBO J Subsets: MEDLINE
    • Publication Information:
      Publication: 2024- : [London] : Nature Publishing Group
      Original Publication: Eynsham, Oxford, England : Published for the European Molecular Biology Organization by IRL Press, [c1982-
    • Subject Terms:
      Arabidopsis*/genetics ; Arabidopsis*/metabolism ; Flowers*/genetics ; Flowers*/metabolism ; Flowers*/growth & development ; Arabidopsis Proteins*/metabolism ; Arabidopsis Proteins*/genetics ; Transcription Factors*/metabolism ; Transcription Factors*/genetics ; CCAAT-Binding Factor*/metabolism ; CCAAT-Binding Factor*/genetics ; DNA-Binding Proteins*/metabolism ; DNA-Binding Proteins*/genetics ; Gene Expression Regulation, Plant*; Photoperiod
    • Abstract:
      Competing Interests: Disclosure and competing interests statement. The authors declare no competing interests.
      The plant master photoperiodic regulator CONSTANS (CO) interacts with Nuclear Factor-Y subunits B2 (NF-YB2) and C9 (NF-YC9) and transcriptionally activates the florigen gene FLOWERING LOCUS T (FT), regulating floral transition. However, the molecular mechanism of the functional four-component complex assembly in the nucleus remains elusive. We report that co-phase separation of CO with NF-YB2/NF-YC9/FT precisely controls heterogeneous CO assembly and FT transcriptional activation. In response to light signals, CO proteins form functional percolation clusters from a diffuse distribution in a B-box-motif-dependent manner. Multivalent coassembly with NF-YC9 and NF-YB2 prevents inhibitory condensate formation and is necessary to maintain proper CO assembly and material properties. The intrinsically disordered region (IDR) of NF-YC9, containing a polyglutamine motif, fine-tunes the functional properties of CO/NF-YB/NF-YC condensates. Specific FT promoter recognition with polyelectrolyte partitioning also enables the fluidic functional properties of CO/NF-YB/NF-YC/FT condensates. Our findings offer novel insights into the tunable macromolecular condensation of the CO/NF-YB/NF-YC/FT complex in controlling flowering in the photoperiod control.
      (© 2024. The Author(s).)
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    • Grant Information:
      E3330900-01 China Science and Technology; 2022B1111230001 key-area research and development program of guangdong province; MOE2019-T3-1-012 Ministry of Education - Singapore (MOE); MOE-T2EP30121-0015 Ministry of Education - Singapore (MOE); MOE-T2EP30122-0021 Ministry of Education - Singapore (MOE); EDUN C-33-18-279-V12 Ministry of Education - Singapore (MOE); MOH-000955 Ministry of Health -Singapore (MOH); NRF-NRFI08-2022-0012 National Research Foundation Singapore
    • Contributed Indexing:
      Keywords: CO/NF-Y; Floral Transition; Macromolecular Condensation; Photoperiodic Regulation
    • Accession Number:
      0 (Arabidopsis Proteins)
      0 (CONSTANS protein, Arabidopsis)
      0 (FT protein, Arabidopsis)
      0 (Transcription Factors)
      0 (CCAAT-Binding Factor)
      0 (DNA-Binding Proteins)
    • Publication Date:
      Date Created: 20241120 Date Completed: 20250103 Latest Revision: 20250105
    • Publication Date:
      20250114
    • Accession Number:
      PMC11696179
    • Accession Number:
      10.1038/s44318-024-00293-0
    • Accession Number:
      39567828