NMR spectroscopy derived plasma biomarkers of inflammation in human populations: Influences of age, sex and adiposity.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Biomarkers*/blood ; Inflammation*/blood ; Adiposity*; Humans ; Female ; Male ; Middle Aged ; Adult ; Aged ; Body Mass Index ; Australia/epidemiology ; Young Adult ; Sex Factors ; Age Factors ; Magnetic Resonance Spectroscopy/methods ; Cohort Studies ; Spain/epidemiology

Understanding the distribution and variation in inflammatory markers is crucial for advancing our knowledge of inflammatory processes and evaluating their clinical utility in diagnosing and monitoring acute and chronic disease. 1H NMR spectroscopy of blood plasma and serum was applied to measure a composite panel of inflammatory markers based on acute phase glycoprotein signals (GlycA and GlycB) and sub-regions of the lipoprotein derived Supramolecular Phospholipid Composite signals (SPC1, SPC2 and SPC3) to establish normal ranges in two healthy, predominantly white cohorts from Australia (n = 398) and Spain (n = 80; ages 20-70 years). GlycA, GlycB, SPC1 and SPC3 were not significantly impacted by age or sex, but SPC2 (an HDL-related biomarker) was significantly higher in women across all age ranges by an average of 33.7%. A free-living Australian population cohort (n = 3945) was used to explore the relationship of BMI with the panel of inflammatory markers. The glycoprotein signals were directly associated with BMI with GlycB levels being significantly higher for women in all BMI classes. Conversely, SPC2 was found to be inversely associated with BMI and differed significantly between the sexes at each BMI category (normal weight p = 3.46x10-43, overweight p = 3.33x10-79, obese p = 2.15x10-64). SPC1 and SPC3 were markedly less affected by BMI changes. Given the significant association between SPC2 and sex, these data suggest that men and women should be modelled independently for NMR-determined inflammatory biomarkers, or that data should be corrected for sex.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2025 Lodge et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Elife. 2022 May 10;11:. (PMID: 35535496)
Clin Chem. 2015 May;61(5):714-23. (PMID: 25779987)
Antioxidants (Basel). 2023 Nov 16;12(11):. (PMID: 38001862)
Anal Chem. 2018 Oct 16;90(20):11962-11971. (PMID: 30211542)
Am Heart J. 1987 Feb;113(2 Pt 2):562-7. (PMID: 3544771)
Am J Med. 1995 Nov;99(5):459-64. (PMID: 7485201)
Nat Commun. 2016 Jan 08;7:10369. (PMID: 26742691)
Commun Med (Lond). 2023 Oct 16;3(1):145. (PMID: 37845506)
Clin Chim Acta. 2016 Jan 15;452:10-7. (PMID: 26549655)
J Proteome Res. 2024 Apr 5;23(4):1328-1340. (PMID: 38513133)
J Proteome Res. 2020 Nov 6;19(11):4428-4441. (PMID: 32852212)
J Lab Precis Med. 2020 Apr;5:. (PMID: 32363327)
Lancet. 2018 Nov 10;392(10159):1736-1788. (PMID: 30496103)
Eur Heart J. 2021 Sep 7;42(34):3388-3403. (PMID: 34333589)
J Infect Dis. 2017 Jul 15;216(2):191-197. (PMID: 28838148)
Atherosclerosis. 1980 Oct;37(2):271-6. (PMID: 7426099)
Anal Chem. 1996 Oct 1;68(19):3370-6. (PMID: 8843135)
Lancet. 2018 May 5;391(10132):1842-1852. (PMID: 29673874)
J Clin Endocrinol Metab. 1989 Jan;68(1):191-9. (PMID: 2909551)
J Lipid Res. 2007 Feb;48(2):328-36. (PMID: 17093293)
Clin Nutr. 2021 Mar;40(3):1241-1246. (PMID: 32847693)
Diabetes Obes Metab. 2019 Jan;21(1):178-182. (PMID: 30047224)
J Clin Lipidol. 2023 Sep-Oct;17(5):677-687. (PMID: 37442713)
PLoS Comput Biol. 2008 May 23;4(5):e1000079. (PMID: 18497853)
PLoS One. 2015 Jan 22;10(1):e0116420. (PMID: 25611320)
PLoS One. 2015 Sep 23;10(9):e0139057. (PMID: 26398105)
Nat Rev Rheumatol. 2023 Jan;19(1):28-43. (PMID: 36418483)
Am J Hematol. 2008 Jun;83(6):435-6. (PMID: 18429053)
Am J Hum Biol. 2012 Jul-Aug;24(4):565-8. (PMID: 22411080)
Metabolites. 2022 Dec 16;12(12):. (PMID: 36557315)
Z Gerontol. 1990 Jan-Feb;23(1):50-5. (PMID: 2327147)
Front Public Health. 2023 Jun 02;11:1105163. (PMID: 37333522)
Semin Arthritis Rheum. 2021 Feb;51(1):219-229. (PMID: 33385862)
Can J Diabetes. 2024 Jun;48(4):244-249.e1. (PMID: 38341135)
J Clin Invest. 2017 Jan 3;127(1):65-73. (PMID: 28045405)
Am J Cardiol. 2023 Oct 1;204:140-150. (PMID: 37542984)
Int J Obes Relat Metab Disord. 1997 Dec;21(12):1115-20. (PMID: 9426378)
Arch Med Sci. 2017 Jun;13(4):851-863. (PMID: 28721154)
Anal Chem. 2022 Mar 15;94(10):4426-4436. (PMID: 35230805)
J Clin Lipidol. 2020 Sep - Oct;14(5):667-674. (PMID: 32863171)
Antioxidants (Basel). 2024 Feb 29;13(3):. (PMID: 38539836)
J Proteome Res. 2024 Aug 2;23(8):2893-2907. (PMID: 38104259)
Eur J Appl Physiol Occup Physiol. 1990;60(6):441-4. (PMID: 2390982)
Biochim Biophys Acta. 2013 Sep;1831(9):1442-8. (PMID: 23792422)
Front Endocrinol (Lausanne). 2022 Jan 10;12:775677. (PMID: 35082753)
Atherosclerosis. 1986 Feb;59(2):131-6. (PMID: 3964340)
J Proteome Res. 2021 Aug 6;20(8):4139-4152. (PMID: 34251833)
Front Nutr. 2023 Dec 04;10:1230480. (PMID: 38111603)
Angew Chem Int Ed Engl. 2023 Aug 28;62(35):e202306154. (PMID: 37341676)
FEBS Lett. 1987 Jul 13;219(1):239-43. (PMID: 3595877)
Anal Chem. 2021 Mar 2;93(8):3976-3986. (PMID: 33577736)
Front Immunol. 2018 Jun 07;9:1302. (PMID: 29951057)
BMC Public Health. 2013 Oct 08;13:936. (PMID: 24099269)
BMC Endocr Disord. 2023 Aug 16;23(1):175. (PMID: 37582770)

0 (Biomarkers)

Date Created: 20250106 Date Completed: 20250106 Latest Revision: 20250108

20250114

PMC11703007

10.1371/journal.pone.0311975

39761295