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HN1 Functions in Protein Synthesis Regulation via mTOR-RPS6 Axis and Maintains Nucleolar Integrity.

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  • Additional Information
    • Source:
      Publisher: Published for the Cell Kinetics Society, the European Study Group for Cell Proliferation, and the International Cell Cycle Society by Blackwell Scientific Publications Country of Publication: England NLM ID: 9105195 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2184 (Electronic) Linking ISSN: 09607722 NLM ISO Abbreviation: Cell Prolif Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Oxford, England] : Published for the Cell Kinetics Society, the European Study Group for Cell Proliferation, and the International Cell Cycle Society by Blackwell Scientific Publications, 1991-
    • Subject Terms:
      TOR Serine-Threonine Kinases*/metabolism ; Cell Nucleolus*/metabolism ; Protein Biosynthesis* ; Ribosomal Protein S6*/metabolism; Humans ; Signal Transduction ; Cell Line, Tumor ; Ribosomes/metabolism ; Cell Cycle ; HeLa Cells
    • Abstract:
      Haematological and Neurological Expressed 1 (HN1) is an oncogene for various cancers and previously has been linked with centrosome clustering and cell cycle pathways. Moreover, HN1 has recently been reported to activate mTOR signalling, which is the regulator of ribosome biogenesis and maintenance. We explored the role of HN1 in mTOR signalling through various gain- and loss-of-function experiments using biochemical approaches in different cell lines. We demonstrated for the first time that HN1 is required for nucleolar organiser region (NOR) integrity and function. Immunoprecipitation-based association and colocalization studies demonstrated that HN1 is an important component of the mTOR-RPS6 axis, and its depletion results with reduced mRNA translation in mammalian cancer cell lines. This study also demonstrated that the depletion of HN1 leads to the irregular distribution of nucleolar structures, potentially leading to cell cycle deregulation as reported previously. Accordingly, components of the translation machinery aggregate with a distinct speckled pattern, lose their essential interactions and ultimately impair mRNA translation efficiency when the HN1 is depleted. These results suggest that HN1 is an essential component of the nucleolus, required for ribosome biogenesis as well as global mRNA translation.
      (© 2025 The Author(s). Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd.)
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    • Grant Information:
      FGA-23342 Ege Üniversitesi; 124Z106 Türkiye Bilimsel ve Teknolojik Araştırma Kurumu; 221Z202 Türkiye Bilimsel ve Teknolojik Araştırma Kurumu
    • Contributed Indexing:
      Keywords: HN1; RPS6; mTOR; nucleolin; nucleolus integrity; ribosome biogenesis
    • Accession Number:
      EC 2.7.11.1 (TOR Serine-Threonine Kinases)
      EC 2.7.1.1 (MTOR protein, human)
      0 (Ribosomal Protein S6)
    • Publication Date:
      Date Created: 20250113 Date Completed: 20250624 Latest Revision: 20250624
    • Publication Date:
      20250624
    • Accession Number:
      PMC12179552
    • Accession Number:
      10.1111/cpr.13805
    • Accession Number:
      39805577