Functional Verification of Differentially Expressed Genes Following DENV2 Infection in Aedes aegypti .

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI

Aedes*/virology ; Aedes*/genetics ; Dengue Virus*/genetics ; Dengue Virus*/physiology ; Virus Replication* ; Mosquito Vectors*/virology ; Mosquito Vectors*/genetics; Animals ; Viral Load ; Gene Expression Profiling ; Salivary Glands/virology ; Salivary Glands/metabolism ; Transcriptome ; Cell Line ; Insect Proteins/genetics ; Insect Proteins/metabolism ; Dengue/virology ; Dengue/transmission

The dengue virus (DENV) is primarily transmitted by Aedes aegypti . Investigating genes associated with mosquito susceptibility to DENV2 offers a theoretical foundation for targeted interventions to regulate or block viral replication and transmission within mosquitoes. Based on the transcriptomic analyses of the midgut and salivary glands from Aedes aegypti infected with DENV2, alongside analyses of Aag2 cell infections, 24 genes potentially related to the regulation of Aedes aegypti infection with DENV2 were selected. By establishing transient transfection and overexpression models of Aedes aegypti Aag2 cells, and mosquito target gene interference models, the difference in viral load before and after treatment was compared, and the effects of DEGs on viral replication were evaluated. After overexpressing 24 DEGs in Aag2 cells, 19 DEGs showed a significant difference in DENV2 RNA copies in the cell supernatant ( p < 0.05). In adult mosquitoes, knocking down defensin-A, defensin-A-like, and SMCT1 respectively reduced the DENV2 RNA copies, while knocking down UGT2B1 and ND4 respectively increased the DENV2 RNA copies. In this study, to assess the role of genes related to DENV2 replication, and transient transfection and overexpression models in Aag2 cells and mosquito gene knockdown models were established, and five genes, defensin-A, defensin-A-like, SMCT1, UGT2B1, and ND4, were found to have an impact on the replication of DENV2, providing a reference basis for studying the complex mechanism of mosquito-virus interactions.

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Keywords: Aag2 cells; Aedes aegypti; DENV2; overexpression; siRNA

0 (Insect Proteins)

Date Created: 20250125 Date Completed: 20250125 Latest Revision: 20250130

20250130

PMC11769442

10.3390/v17010067

39861856