Transcriptomic and metabolomic study of the biosynthetic pathways of bioactive components in Amomum tsaoko fruits.

Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE

Original Publication: London : BioMed Central, [2001-

Fruit*/metabolism ; Fruit*/genetics ; Biosynthetic Pathways*/genetics ; Amomum*/metabolism ; Amomum*/genetics ; Transcriptome*; Terpenes/metabolism ; Metabolomics ; Gene Expression Regulation, Plant ; Curcumin/metabolism ; Transcription Factors/metabolism ; Transcription Factors/genetics ; Gene Expression Profiling

Competing Interests: Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
Amomum tsaoko is a significant medicinal and edible plant with documented efficacy in the treatment of various diseases. Additionally, it is a crucial food additive and spice. 1,8-cineole and curcumin are the main bioactive compounds of A. tsaoko, and research on these compounds has mainly focused on their chemical composition and pharmacological activity, with relatively less exploration of synthetic pathways and identification of key genes. This study employed transcriptome sequencing and metabolomic analysis of A. tsaoko at five different developmental stages (May fruit - September fruit) to assess the accumulation patterns of terpenoid and curcuminoid compounds and to explore the key genes and transcription factors (TFs) involved in their synthesis pathways. The results showed that three genes encoding 1-deoxy-D-xylulose-5-phosphate synthase (DXS), hydroxymethylglutaryl-CoA synthase (HMGCS) and phosphomevalonate kinase (mvaK2) and TFs such as AP2-ERF, bHLH, WRKY were screened for involvement in terpenoid biosynthesis. In addition, three genes encoding trans-cinnamate 4-monooxygenase (C4H), curcumin synthase (CURS) and TFs such as MYB, bHLH, bZIP were screened for involvement in curcuminoid biosynthesis. This study provides a theoretical foundation for further research into the biosynthesis of active components in A. tsaoko, establishing a basis for in-depth investigations into the mechanisms underlying its medicinal quality formation. Additionally, it offers guidance for the utilisation of its aromatic components and natural pigments.
(© 2025. The Author(s).)

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82260739, 32260094 National Natural Science Foundation of China; 202101AZ070001-005 the Yunnan Provincial Science and Technology Department - Applied Basic Research Joint Special Funds of Yunnan University of Traditional Chinese Medicine

Keywords: Amomum tsaoko; Curcuminoids; Metabolomics; Terpenoids; Transcriptional Regulation; Transcriptomics

0 (Terpenes)
IT942ZTH98 (Curcumin)
0 (Transcription Factors)

Date Created: 20250218 Date Completed: 20250508 Latest Revision: 20250508

20250509

PMC11834249

10.1186/s12870-025-06239-w

39966750