The Application of DNA Viruses to Biotechnology.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI

Biotechnology*/methods ; DNA Viruses*/genetics ; DNA Viruses*/physiology; Humans ; Genetic Engineering ; Animals

The delivery of biomolecules to target cells has been a longstanding challenge in biotechnology. DNA viruses naturally evolved the ability to deliver genetic material to cells and modulate cellular processes. As such, they inherently possess requisite characteristics that have led to their extensive study, engineering, and development as biotechnological tools. Here, we overview the application of DNA viruses to biotechnology, with specific implications in basic research, health, biomanufacturing, and agriculture. For each application, we review how an increasing understanding of virology and technological methods to genetically manipulate DNA viruses has enabled advances in these fields. Additionally, we highlight the remaining challenges to unlocking the full biotechnological potential of DNA viral technologies. Finally, we discuss the importance of balancing continued technological progress with ethical and biosafety considerations.

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1U01MH130700 United States NH NIH HHS; 1R01NS126397 United States NH NIH HHS

Keywords: adeno-associated virus; adenovirus; agriculture; gene therapy; herpesvirus; microbiome editing; oncolytic virotherapy; phage therapy; vaccine; vaccinia virus

Date Created: 20250327 Date Completed: 20250515 Latest Revision: 20250709

20250710

PMC11946468

10.3390/v17030414

40143341