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Epitope Mapping of Senecavirus A 3A Protein Using Monoclonal Antibodies.

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  • Additional Information
    • Source:
      Publisher: Blackwell Verlag Country of Publication: Germany NLM ID: 101319538 Publication Model: eCollection Cited Medium: Internet ISSN: 1865-1682 (Electronic) Linking ISSN: 18651674 NLM ISO Abbreviation: Transbound Emerg Dis Subsets: MEDLINE
    • Publication Information:
      Original Publication: Berlin : Blackwell Verlag
    • Subject Terms:
      Epitope Mapping*/veterinary ; Antibodies, Monoclonal*/immunology ; Picornaviridae*/immunology ; Picornaviridae*/genetics ; Picornaviridae Infections*/veterinary ; Picornaviridae Infections*/virology ; Picornaviridae Infections*/immunology ; Swine Diseases*/virology; Animals ; Mice ; Mice, Inbred BALB C ; Swine ; Antibodies, Viral/immunology ; Amino Acid Sequence
    • Abstract:
      Competing Interests: The authors declare no conflicts of interest.
      Senecavirus A (SVA), an emerging pathogen causing vesicular disease in pigs, poses a significant threat to the swine industry. The nonstructural protein 3A of SVA plays an essential role in the viral replication cycle. In this study, we immunized mice with the prepared SVA 3A protein and produced two monoclonal antibodies (mAbs), AG4 and 2F3. MAb AG4 showed specific reactivity to the linear and conformational 3A protein, whereas mAb 2F3 did not recognize linear epitope of 3A protein. Through truncated 3A protein expression and alanine mutation analysis, we identified 1 SPNEND 6 as the minimal motif recognized by mAb AG4, with Asn 3 being the critical residue. Additionally, we demonstrated that mAb 2F3 failed to recognize the SVA mutant with the 75 QEETEG 80 deletion in 3A protein, indicating that 75 QEETEG 80 constitutes an essential epitope for mAb 2F3. Further deletion analysis confirmed that 75 QE 76 is the crucial motif for mAb 2F3 recognition. Moreover, we found that 1 SPNEND 6 and 75 QEETEG 80 are highly conserved among different SVA strains and are exposed on the surface of the 3A protein. This study contributes to further explore the function of SVA 3A protein and develop diagnostic tools for SVA detection.
      (Copyright © 2025 Liang Meng et al. Transboundary and Emerging Diseases published by John Wiley & Sons Ltd.)
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    • Contributed Indexing:
      Keywords: 3A protein; Senecavirus A; epitope; monoclonal antibody
    • Accession Number:
      0 (Antibodies, Monoclonal)
      0 (Antibodies, Viral)
    • Subject Terms:
      Senecavirus A
    • Publication Date:
      Date Created: 20250515 Date Completed: 20250515 Latest Revision: 20250516
    • Publication Date:
      20250519
    • Accession Number:
      PMC12077972
    • Accession Number:
      10.1155/tbed/3398924
    • Accession Number:
      40371172