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Teaching CRISPR-Cas9 Genome Editing through Novel Research in a Multi-Week Lab Module

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  • Author(s): Nannas, Natalie J.
  • Language:
    English
  • Source:
    Bioscene: Journal of College Biology Teaching. Dec 2021 47(2):14-20.
  • Publication Date:
    2021
  • Document Type:
    Journal Articles
    Reports - Descriptive
  • Additional Information
    • Availability:
      Association of College and Biology Educators. Web site: http://acube.org
    • Peer Reviewed:
      Y
    • Source:
      7
    • Sponsoring Agency:
      National Science Foundation (NSF)
    • Contract Number:
      MCB1925546
    • Intended Audience:
      Teachers
    • Education Level:
      Higher Education
      Postsecondary Education
    • Subject Terms:
      Genetics; Science Instruction; Undergraduate Students; Scientific Research; Laboratory Experiments; Molecular Biology; Computer Software; Computer Assisted Instruction
    • ISSN:
      1539-2422
    • Abstract:
      CRISPR-Cas9 is revolutionizing how we conduct scientific research, treat disease, and develop new crops. The widespread impact of this genome-editing technology makes it critical for undergraduate students to understand and engage with this new tool. In this article, we describe a multi-week lab module that teaches undergraduates how to design CRISPR-Cas9 constructs and screen for CRISPR-modified genotypes. The module is conducted through the lens of independent research; students conduct a genotype screen for novel knockout mutations. In our module, students screen Zea mays (maize) seedlings for mutations in the MAD2 gene, which assists our ongoing investigation of meiotic chromosome segregation. This module can be adapted to knockout any gene in any organism, and thus align with an instructor's research program. Engaging in original research helps undergraduate students develop independence and initiative in the lab as well as the molecular techniques of CRISPR-Cas9.
    • Abstract:
      As Provided
    • Publication Date:
      2022
    • Accession Number:
      EJ1339379