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Kinetics of Dengue Non-Structural Protein 1 Antigen and IgM and IgA Antibodies in Capillary Blood Samples from Confirmed Dengue Patients

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  • Additional Information
    • Contributors:
      Centre National de Référence pour les Arbovirus - Laboratoire de Virologie [Cayenne, Guyane française] (CNR - laboratoire associé); Institut Pasteur de la Guyane; Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP); Far East Medical Vietnam Limited [Ho Chi Minh City, Vietnam] (FV Hospital); Institut Pasteur d'Ho Chi Minh Ville; Réseau International des Instituts Pasteur (RIIP); Laboratoire des Interactions Virus-Hôtes [Cayenne, Guyane Française]; Centre d'épidémiologie et de santé publique des armées [Marseille] (CESPA); Service de Santé des Armées; Centre de Recherche des Cordeliers (CRC (UMR_S 872)); Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS); This study was supported by Conseil Regional de la Guyane Agreement 60/2007/CR; The authors thank all patients for their participation in this study. The authors also thank Prof. Nguyen Tran Chinh (Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam) and Dr. Nguyen Quoc Khanh and Dr. Phan Ngoc Nam (People's Hospital 115, Ho Chi Minh City, Vietnam) for their help and support during this study.; Centre National de Référence pour les Arbovirus - Laboratoire associé de Virologie (CNR); Centre d'épidémiologie et de santé publique des armées (CESPA)
    • Publication Information:
      HAL CCSD, 2014.
    • Publication Date:
      2014
    • Abstract:
      International audience; Large-scale epidemiological surveillance of dengue in the field and dengue patient management require simple methods for sample collection, storage, and transportation as well as effective diagnostic tools. We evaluated the kinetics of three biological markers of dengue infection-non-structural protein 1 (NS1) antigen, immunoglobulin M (IgM), and IgA-in sequential capillary blood samples collected from fingertips of confirmed dengue patients. The overall sensitivities and specificities of the tests were 96% and 100%, respectively, for NS1, 58.1% and 100%, respectively, for IgM, and 33% and 100%, respectively, for IgA. During the acute phase of the disease, NS1 was the best marker of dengue infection, with a sensitivity of 98.7%, whereas from day 5, all three markers exhibited relevant levels of sensitivity. This first descriptive study of the kinetics of biological markers of dengue in capillary blood samples confirms the usefulness of this biological compartment for dengue diagnosis and argues for its exploitation in community-level and remote settings.
    • ISSN:
      0002-9637
    • Accession Number:
      10.4269/ajtmh.13-0458⟩
    • Rights:
      OPEN
    • Accession Number:
      edsair.doi.dedup.....a44ad62c05399b9749b731119b7fb9f9