Consequences of high-protein diets consumption, anabolic androgenic steroids administration and high-intesity exercise on brain and kidney oxidative stress markers in wistar rats ; Consecuencias del consumo de dietas hiperprotéicas, de la administración de anabolizantes androgénicos esteroideos y de la práctica de un entrenamiento de alta intensidad sobre marcadores de estrés

The overall objective of this PhD Thesis was to analyse the brain and kidney antioxidant defence system and oxidative damage as well as the renal morphology effects of high-protein diet, high-intensity exercise, and anabolic androgenic steroids administration in Wistar rats. The main findings from this Thesis suggest that: 1) High-protein diets may cause oxidative damage to the brain by means of lipid and protein oxidation, which could explain the induction of the endogenous antioxidant defence system. 2) High-intensity exercise protocol did not worsen the deleterious effects caused by high-protein diet and may be an efficient way to protect the brain against high dietary protein aggression. 3) High-protein diets led to a prooxidant status at kidney level. However, the beneficial effect of high-intensity exercise observed on brain, did not appear at kidney level. 4) The high-intensity exercise protocol displayed a worse renal morphological profile, which might be associated with a higher risk for incidence of kidney disease in the long-term. The stress induced by the type of exercise performed in the present Thesis could be related to this worse morphological renal status. 5) Under our experimental conditions, the present results suggest that high-intensity exercise reduce the negative effects of anabolic androgenic steroids on brain redox status. High-intensity exercise also improved the harmful effects caused by the anabolic androgenic steroids administration on kidney lipid and protein oxidation. The results of the current Thesis underline that high-protein diets intake and the anabolic androgenic steroids administration instigated brain and kidney damage by means of the induction of lipid and protein oxidation. Despite the apparently beneficial effect of high-intensity exercise among the others two interventions studied, cautiousness should be taken with this protocol regarding to brain and kidney overproduction of their antioxidant defence systems. ; El objetivo general de esta Tesis Doctoral ha sido ...