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Molecular identification and phylogenetic analysis of Trypanosoma evansi in dromedaries (Camelus dromedarius) from Iran ; Molekularna identifikacija i filogenetska analiza Trypanosoma evansi u jednogrbih deva (Camelus dromedarius) iz Irana

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  • Additional Information
    • Publication Date:
      2021
    • Collection:
      Hrčak - Portal of scientific journals of Croatia / Portal znanstvenih časopisa Republike Hrvatske
    • Abstract:
      Surra is of great concern to countries in the world such as Iran, which have a considerable camel population. The present study was aimed at determining the prevalence of Trypanosoma evansi in the camels of Iran. A total of 167 blood samples from farmed camels were examined for the presence of T. evansi infection using parasitological and molecular methods. Blood smear examination revealed 10 (6%) positive samples, while the PCR method 14 (8.4%) found positive samples. Age, sex, and region were not determined as risk factors for T. evansi infection in this study. The phylogenetic tree inferred from VSG gene sequences of T. evansi clearly separated the sequences of this study into two clades, A and B, which reflects the intrasequence heterogeneity among Iranian isolates. The phylogenetic tree showed that Iranian T. evansi strains are members of the T. brucei clade. ; Sura je bolest od posebne važnosti u zemljama kao što je Iran, koje imaju veliku populaciju deva. Cilj ovoga istraživanja bio je odrediti prevalenciju Trypanosoma evansi u deva u Iranu. Ukupno 167 uzoraka krvi farmski držanih deva istraživano je na prisutnost invazije s T. evansi primjenom parazitoloških i molekularnih metoda. Istraživanje krvnog razmaza pokazalo je 10 (6 %) pozitivnih uzoraka, dok je PCR pokazao 14 (8,4 %) pozitivnih uzoraka. U ovom istraživanju dob, spol i područje uzgoja nisu određivani kao rizični čimbenici za invaziju s T. evansi. Filogenetsko stablo izvedeno iz sekvencija VSG gena T. evansi jasno je razdvojilo sekvencije u dvije klade, A i B, i odražava unutarsekvencijsku heterogenost među iranskim izolatima. Filogenetsko stablo pokazalo je da su iranski sojevi T. evansi članovi klade T. brucei.
    • File Description:
      application/pdf
    • Relation:
      https://hrcak.srce.hr/260231
    • Online Access:
      https://doi.org/10.24099/vet.arhiv.1004
      https://hrcak.srce.hr/260231
      https://hrcak.srce.hr/file/378492
    • Rights:
      info:eu-repo/semantics/openAccess ; Full-text papers can be used for personal or educational purposes only. Authors' and publisher's copyrights must be acknowledged.
    • Accession Number:
      edsbas.16853956