Data from: Self-extinguishing relay waves enable homeostatic control of human neutrophil swarming

Neutrophils collectively migrate to sites of injury and infection. How these swarms are coordinated to ensure the proper level of recruitment is unknown. Using an ex vivo model of infection, we show that human neutrophil swarming is organized by multiple pulsatile chemoattractant waves. These waves propagate through active relay in which stimulated neutrophils trigger their neighbors to release additional swarming cues. Unlike canonical active relays, we find these waves to be self-terminating, limiting the spatial range of cell recruitment. We identify an NADPH-oxidase-based negative feedback loop that is needed for this self-terminating behavior. We observe near-constant levels of neutrophil recruitment over a wide range of starting conditions, revealing surprising robustness in the swarming process. This homeostatic control is achieved by larger and more numerous swarming waves at lower cell densities. We link defective wave termination to a broken recruitment homeostat in the context of human chronic granulomatous disease. ; Funding provided by: American Heart Association Crossref Funder Registry ID: https://ror.org/013kjyp64 Award Number: Funding provided by: National Institute of General Medical Sciences Crossref Funder Registry ID: https://ror.org/04q48ey07 Award Number: GM118167 Funding provided by: National Science Foundation Crossref Funder Registry ID: https://ror.org/021nxhr62 Award Number: 2019598 Funding provided by: National Science Foundation Crossref Funder Registry ID: https://ror.org/021nxhr62 Award Number: DBI-1548297 Funding provided by: Novo Nordisk Foundation Crossref Funder Registry ID: https://ror.org/04txyc737 Award Number: NNF17OC0028176 ; Neutrophil Isolation Protocol: Imaging media was first prepared with RPMI (w/o Phenol Red, 25mM HEPES, L-Glutamate) and 0.4% Human Serum Albumin (HSA) (Sigma Cat: A5843). HSA was added directly to RPMI and then centrifuged at 500xg for 5 min until fully dissolved. The mix was then filtered with a .22 μm Steriflip filter (Millipore Sigma: SE1M179M6) ...