Bleomicino pernaša į navikines ląsteles in vivo ir navikų gydymas taikant elektroporaciją ir sonoporaciją ; Bleomycin transfer into tumor cells in vivo and tumor treatment using electroporation and sonoporation methods

There is a lot of various information about cancer as well as plenty of knowledge and types of drugs to treat this disease. Pharmacy has discovered and developed a number of active anti-cancer chemotherapeutic agents for cancer treatment, or at least useful for reducing the spread of cancer. Therefore, another important and urgent problem is development of new method that would help to deliver directly into cancer cells or tissue. This in turn would allow to reduce side effects of the drug and preserve healthy tissues and organs. In this experimental work we attempted to answer this problem, using two methods that facilitate the transfer of materials into the cells - electroporation and sonoporation. These methods are based on similar principle: both electroporation and sonoporation affects the cell membranes to form pores that allow for various products (medicines, dyes, proteins, genes) to get access into the cell. The cell naturally is not accessible for those materials or the permeability is very small, in case special or nonspecific transport systems exist for this specific molecule. In this study we used the following two methods in order to facilitate the anticancer drug bleomycin delivery into cancer cells in vivo. The subject of these experiments in vivo is CBA – line clone mice bearing MH22A hepatoma tumors, transplanted on upper part of the flank. Experimental data showed that both electroporation and sonoporation and combination of both methods are effective in treating tumors. These methods facilitate the chemotherapeutic drugs, in our case, transport of bleomycin to tumor cells. All three conditions used: only electroporation, only sonoporation as well as combination of electroporation and sonoporation resulted a noticeable decrease in tumor volume size. Moreover in some case mice were cured without any tumor recurrence during sever postreatment months. It appeared that sonoporation was more aggressive than electroporation; after sonoporation treatment tumors decreased more rapidly, however the ...